Relation of gene expression to anthocyanins metabolism in strawberry fruits
The aim of this research is to probe further into the anthocyanins metabolism and functions of enzymes involved in the synthesis of anthocyanins in strawberry fruits. The coding sequences of three genes encoding DFR.ANS and LAR were cloned from 'Albion' (Fragaria x ananassa ) strawberry fruits by RT-PCR. The predicted protein sequences shared 99%～100% homology to those of 'Queen Elisa' and ' Korona' ( Fragaria x ananassa) strawberry published in NCBI.Semiquantitative RT-PCR showed that DFR had three transcript level peaks at fruitlet stage, white stage and red stage, respectively, with the highest at red stage. The transcript level of ANS increased rapidly from white stage,reaching the highest at red stage. The transcript level of LAR showed a decreased trend with the highest at fruitlet stage. During the development of strawberry fruits, the transcript level of DFR peaks at fruitlet and red stages correlated with the concentration of flavan-3-nols and anthocyanins. The LAR and ANS were closely correlative with the synthesis of anthocyanins and flavan-3-ols,respectively.
Fragaria x ananassagene expressionanthocyanins metabolism
国家科技期刊平台
NSTL
万方数据
维普
