Antibacterial effects of Chinese herbal medicines on bovine pathogenic Escherichia coli in vitro
The aim of this study was to study the antibacterial effects of 16 Chinese herbal medicines on 5 different serotypes of pathogenic Escherichia coli (O1、O2、O8、O78 and O86) in vitro.The diameter of inhibition zone was determined by Oxford cup method.Micro dilution and plate method were determined by minimal inhibitory concentration (MIC) and minimum bactericidal concentration (MBC).1)Lonicera japonica and Coptis chinensis had the best inhibitory effects on E.coli O1,and the inhibition zones were 29.35 and 37.71 mm respectively.Schisandra chinensis had the best inhibitory effect on E.coli O8 the inhibition zone was 28.97 mm.2)The MIC of L.japonica and C.chinensis on E.coli O1 were 0.016 and 0.008 g/mL respectively.The MIC of S.chinensis on E.coli O8 was 0.008 g/mL.All herbs displayed better inhibitory effects.3) Polygonum cuspidatum had the worst inhibitory effects,inhibition zone,MIC and MBC were not detected.The inhibition zone of L.japonica,C.chinensis and S.chinensis on 5 different serotypes were above 15 mm and reached significantly sensitive,Radix sanguisorbae,Andrographic,P.cuspidatum,R.bupleuri,golden cypress and Pomegranate husk were not sensitive.L.japonica,C.chinensis,P.multiflorum,R.sanguisorbae,S.chinensis and Cortex fraxini displayed better inhibitory effects on 5 different serotypes.
Chinese herbspathogenic Escherichia coliMICMBCantibacterial in vitro
国家科技期刊平台
NSTL
万方数据
维普
