523份玉米自交系对禾谷镰孢穗腐病和灰斑病的抗性鉴定
Resistance evaluation of 523 maize inbred lines to Gibberella ear rot and gray leaf spot
陈鸽 1王敏 2周德龙 1刘宏伟 1张志军 1苏前富 3卢实 1焦仁海 1夏远峰 1徐国良 1刘文国 4路明1
作者信息
- 1. 吉林省农业科学院(中国农业科技东北创新中心)玉米研究所/国家玉米工程技术研究中心(吉林)/农业农村部东北中部玉米生物学与遗传育种重点实验室,长春 130033
- 2. 吉林吉农高新技术发展股份有限公司,吉林公主岭 136100
- 3. 吉林省农业科学院(中国农业科技东北创新中心)植物保护研究所,长春 130033
- 4. 主要粮食作物国家工程研究中心,长春 130033
- 折叠
摘要
为筛选东北地区抗禾谷镰孢穗腐病和灰斑病的玉米自交系,2021-2023和2022-2023年分别对523份玉米自交系开展了禾谷镰孢穗腐病和灰斑病的田间人工接种抗性鉴定,试验采用随机区组设计,在吐丝10d后和9-11叶期通过针刺接种法和心叶灌注法分别接种禾谷镰孢穗腐病和灰斑病的病菌,在蜡熟末期采用单株调查法对禾谷镰孢穗腐病病情级别进行调查,在乳熟后期对灰斑病病情发生级别进行调查.结果表明:在禾谷镰孢穗腐病的抗性鉴定中,'PHRE 1'3年均稳定表现抗病,'丹598''吉A1473''LN 1'等23份自交系在3年内均达到中抗以上,占总参试自交系的4.2％;在灰斑病的抗性鉴定中,'丹299'在2年中均稳定表现出抗病,'LH217'、'吉1037'和'吉A1001'等67份自交系2年中均达到中抗以上,占总参试自交系的12.6％.综上,'吉A1473'、'A-349'和'M 67'这3个自交系对2种病害均能达到中抗以上,可用于培育抗禾谷镰孢穗腐病和抗灰斑病的春玉米新种质.
Abstract
In order to identify maize inbred lines resistant to Gibberella ear rot and gray leaf spot in the northeast region,a precise evaluation of 523 maize inbred lines resistance to Gibberella ear rot and gray leaf spot was carried out by field inoculation from 2021 to 2023 and 2022 to 2023,respectively.The experiment adopted a randomized block design and inoculated Gibberella ear rot pathogen through the needle puncture inoculation method after 10 d of silking and gray leaf spot pathogen through the perfusion methods after 9-11 leaves stage.At the wax ripeness stage,the single plant survey method was adopted to investigate the severity level of Gibberella ear rot disease.At the late milk stage,the whole-line survey method was adopted to investigate the severity level of gray leaf spot disease.The results showed that:The inbred line'PHRE1'was stably resistant to Gibberella ear rot from 2021 to 2023.Twenty-three inbred lines including'Dan 598','JiA 1473'and'LN 1'exhibited moderate resistance or above within three years,accounting for 4.2％of the total materials.The inbred line'Dan 299'was resistant to gray leaf spot disease for two years.Sixty-seven inbred lines including'LH 217','Ji 1037'and'JiA1001'expressed moderate resistance or above in two years,accounting for 12.6％of the total test materials.By comparison analysis,a total of three inbred lines('JiA1473','A-349'and'M67')identified were resistant to the two diseases.This study provided valuable resources and a scientific basis for the cultivation and improvement of resistant maize varieties.
关键词
玉米/自交系/抗性鉴定/禾谷镰孢穗腐病/灰斑病Key words
maize/inbred lines/resistance identification/Gibberella ear rot/gray leaf spot引用本文复制引用
出版年
2025
国家科技期刊平台
NSTL
万方数据