Bioinformatics and expression analysis of XTH gene in Agropyron mongolicum Keng under drought stress
To screen the XTH genes related to drought resistance in Agropyron mongolicum Keng,the physical and chemical properties,subcellular localization,conserved motif,evolutionary relationship and secondary and tertiary structures were analyzed by bioinformatics methods,and the expression of four XTH genes related to drought resistance was monitored.The results showed that:The seven XTH genes of A.mongolicum were hydrophilic proteins with molecular weight of 7.67 to 39.46 ku,the theoretical isoelectric point(PI)of 4.67 to 9.81,and protein size of 70 to 357 aa.Subcellular localization analysed showed that AmXTH-1 was located in the cell wall and cytoplasm,and the rest 6 genes were located in the cell wall.The secondary and tertiary structures of the XTH gene were mainly random coils,and motif 1,motif2 and motif3 were speculated to be conserved motifs.Phylogenetic analysis showed that AmXTH30,AmXTH28-1,AmXTH-1 and AmXTH8-1 had high similarity with known genes with drought resistance.The qRT-PCR analysis showed that the four XTH genes related to drought resistance of A.mongolicum were down-regulated.It is speculated that the four differentially expressed XTH genes in A.mongolicum play a negative regulatory role in response to drought stress.
国家科技期刊平台
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