Diversity and structure of arbuscular mycorrhizal fungi communities in rhizosphere soil of different wine grape varieties in the eastern foot of Helan Mountain
The purpose of this study was to clarify the colonization and community diversity of symbiotic Arbuscular mycorrhizal fungi(AMF)in wine grapes.The roots and rhizosphere soil samples at a range of soil depths(0-20,20-40,and 40-60 cm)of three major wine grape varieties(Cabernet Sauvignon,Merlot,and Chardonnay)were collected from the eastern foot of Helan Mountain in Ningxia,China.Acetic acid-ink staining method was used to observe the AMF infection status of wine grape roots.Illumina MiSeq high-throughput sequencing technology was used to comprehensively analyze the composition and diversity of AMF communities in the rhizosphere of wine grapes.The results showed that:AMF could establish a good symbiotic relationship with wine grapes,colonize in grape roots and form Arum-type structure.A total of 64 operational taxonomic units(OTUs)were obtained and further classified into 1 phylum,3 classes,5 orders,7 families,7 genera,and 32 species.Cabernet Sauvignon had the highest AMF community richness in rhizosphere soil,and Merlot had the highest community diversity,richness,and evenness,and Chardonnay had the highest pedigree diversity.The community diversity,richness,and evenness of AMF in the rhizosphere soil of 0 to 20 cm were the highest,and the pedigree diversity of 20 to 40 cm was the highest.Glomus was the dominant genera of AMF among 3 varieties and 3 soil depths.Only Chardonnay recognized AMF biomarkers in different soil depths.Variety was the main factor affecting the variation of the AMF community in the rhizosphere soil of wine grapes.In conclusion,this research provides a firm foundation for future exploration on the beneficial use of AMF in enhancing grapevine production,quality,and sustainability.
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