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210份板栗种质资源坚果表型性状多样性分析

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为解析中国板栗种质资源坚果表型性状多样性,对11个省市210份中国板栗资源的19个表型性状(2个假性质量性状、5个质量性状和12个数量性状)进行测定,利用Shannon-Wiener's多样性指数评价表型性状的遗传多样性,并综合运用相关性、主成分和聚类分析等方法对坚果表型进行多样性分析。结果表明:210份资源的19个坚果表型性状具有较高的多样性水平,变异系数介于4。38%~47。98%,遗传多样性指数介于0。45~1。96,其中数量性状的遗传多样性指数普遍高于质量性状;坚果表型性状群体间变异约占总变异的1/4,群体内约占3/4,群体内变异是坚果表型变异的主要来源;出仁率与单粒质量、种仁质量呈极显著正相关,果形指数只与坚果长呈极显著正相关,与其他性状呈极显著负相关(P<0。01);主成分分析将12个数量性状转换为3个独立的综合指标,累计贡献率达87。334%,最能反应坚果表型特征的3个性状是坚果长、种仁质量和单粒质量;基于遗传距离聚类,供试资源划分为4大类群,各类群间表型性状差异明显,第Ⅲ-1亚类资源普遍单粒质量较大、出仁率相对较高,可作为大粒加工型良种培育优良亲本。本研究从多角度系统分析了 210份板栗资源坚果表型性状的多样性,可为优异种质的挖掘及创新利用提供参考。
Diversity analysis of nut phenotypic traits of 210 Chinese chestnut germplasm resources
To analysis the nut phenotypic traits diversity of Chinese chestnut,a total of 19 phenotypic traits(two false qualitative traits,5 qualitative traits and 12 quantitative traits)of 210 Chinese chestnut resources from 11 provinces were determined.Then genetic diversity of nut phenotypic traits was calculated based on the Shannon-Wiener information diversity index.The chestnut germplasms were comprehensively evaluated by using the correlation analysis,principal component and cluster analysis.The results showed that:The 19 nut phenotypic traits of 210 chestnut resources displayed a high level of phenotypic diversity,with variation coefficients ranged from 4.38%to 47.98%,and the genetic diversity index ranged from 0.45 to 1.96.The diversity index of quantitative traits was generally higher than that of qualitative traits.The nut phenotypic variation among populations accounted for about 1/4 of the total variation,and about 3/4 within populations.Correlation analysis showed that kernel rate was significantly positively correlated with nut size and kernel weight.Nut shape index was only significantly positively correlated with nut length,and was significantly negatively correlated with other traits(P<0.01).Principal component analysis showed that the 12 quantitative traits were integrated into three principal components,and their additive contributing rate reached 87.334%.The three traits that could best reflect the phenotypic characteristics of nuts were the nut length,kernel weight and nut size.The 210 germplasm resources were systematically clustered into four groups based on the genetic distance,and there were significant differences among the groups.The germplasms in group Ⅲ-1 could be used as excellent parents for material innovation and hybrid breeding because of their large nut size and high kernel rate.The diversity of nut phenotypic traits of Chinese chestnut was systemically analyzed from multiple perspectives in this study,which could provide a scientific basis for the discovery and innovative utilization of excellent chestnut germplasm.

Chinese chestnutnut phenotypesdiversitycomprehensive evaluation

张馨方、郭燕、张树航、李颖、刘金雨、范丽颖、王广鹏

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河北省农林科学院昌黎果树研究所,河北昌黎 066600

板栗 坚果表型 多样性 综合评价

2025

中国农业大学学报
中国农业大学

中国农业大学学报

北大核心
影响因子:0.971
ISSN:1009-508X
年,卷(期):2025.30(1)