Comparative analysis of transcriptomes between coarse and fine hair follicles in Angora rabbits
To screen for genes and regulatory pathways that affect the diameter of Angora rabbit hair fibers,RNA sequencing(RNA-Seq)was used to identify expressed genes in the coarse and fine hair follicles of Angora rabbits,and differentially expressed genes(DEGs)functional enrichment was performed.Additionally,a protein-protein interaction(PPI)network was constructed using Cytoscape to identify hub genes involved in regulating the diameter of rabbit hair fibers.Finally,quantitative real-time PCR(qRT-PCR)was employed for validation of the DEGs.The results showed that:1)A total of 412 DEGs were identified by RNA-seq.Compared to coarse hair follicles,160 DEGs were up-regulated in fine hair follicles and 252 DEGs were down-regulated.2)GO functional analysis showed that differentially expressed genes were significantly enriched in 330 biological processes,including molting cycle hair cycle,hair cycle regulation,supramolecular fiber formation,and other articles related to the growth of coarse and fine hair.Additionally,KEGG enrichment analysis demonstrated that differentially expressed genes were significantly enrichment in the estrogen signaling pathway,protein digestion and absorption pathway,endoplasmic reticulum protein processing pathway,PPAR signaling pathway,FoxO signaling pathway,and basal cell carcinoma pathway,which were related to the growth and differentiation of coarse and fine hair in Angora rabbits.3)Protein interaction network analysis highlighted SHH,LGR5,CTSB,ACTA2,and CRNN as important functional genes regulating the structure of hair fibers.In conclusion,this study elucidated the molecular mechanism underlying the regulation of rabbit hair fineness,identified DEGs involved in controlling rabbit hair diameter,and discovered 5 potential candidate genes associated with coarse and fine hair differentiation in Angora rabbits.These findings provide a solid theoretical foundation and empirical evidence to support the breeding program of fine-haired Angora rabbits.
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