Vitrification phenomenon exploration and physiological characteristics analysis of strawberry in vitro seedlings
In order to investigate the vitrification phenomenon and physiological characteristics of strawberry test-tube seedlings,eight test-tube seedling varieties,'Benihoppe','Yuexiu','Red Pearl','Ssanta','Light Spot','Snow White','Miaoxiang'and'Akihime'were taken as test materials to analyze the vitrification difference of different varieties of strawberry test-tube seedlings.By measuring the moisture content,relative content of chlorophyll,relative conductivity,malondialdehyde content and oxidase activity of the vitrification test tube seedling of'Benihoppe'strawberry,the physiological characteristics of the vitrification test tube seedling were further analyzed.In addition,the effects of content and treatment time of HgCl2 on the vitrification of test-tube seedlings and the differences of physiological traits of test-tube seedlings with different degrees of vitrification after acclimation and transplanting were investigated after domestication and transplanting.The results showed that:1)Different strawberry varieties have different vitrification rates.Among these,the'Snow White'strawberry had the highest vitrification rate of 31.0％,while the'Ssanta'strawberry had the lowest vitrification rate of 14.0％.2)The water content,relative electrical conductivity and malondialdehyde content of vitrification strawberry test-tube seedlings were significantly higher than those of normal strawberry test-tube seedlings,while the plant height and chlorophyll content were significantly lower than those of normal strawberry test-tube seedlings(P＜0.05).3)The induction catalase(CAT)activity of vitrification strawberry tube seedlings decreased significantly,and the activity of peroxidase(POD)increased significantly(P＜0.05).4)The higher the content of HgCl2,the longer the disinfection time,and the higher the vitrification rate.After treatment with 0.5％HgCl2 for 16 min,the vitrification rate of'Benihoppe'strawberry reached 38.2％.5)After the domestication and transplanting of severe vitrification strawberry test tube seedlings,the plant height,relative chlorophyll content,thick stem,dry and fresh weight were significantly lower than those of normal strawberry root seedlings(P＜0.05).In conclusion,the vitrification of strawberry test tube seedlings may be an oxidative stress reaction.
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NSTL
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