Identification and Degradation Optimization of a High Nicotine Endurance Strain Arthrobacter sp.AH14
Nicotine is the main component of tobacco alkaloids,its content will directly affect the quality of tobacco leaves.At present,our country's cigarette enterprises face a hard problem that upper leaves of high nicotine content can't be greatly used in tobacco blend.Besides,since nicotine is toxic,how to remove nicotine from tobacco waste is another major challenge which tobacco industry faces,so it's very important to find a simple,low consumption,environmental approach to degrade nicotine.Our laboratory previously isolated and stored a nicotine degrading strain AH14 and this study identified AH14 as Arthrobacter sp.,according to the morphological characteristics,biochemical and physiological property,16S rDNA sequencing and phylogenetic tree analysis.Then,with 6 g/L nicotine as the sole carbon source,the nicotine degradation conditions of this strain was optimized,the results showed that the optimum conditions for nicotine degradation were:inoculum size 12.5％,pH 6.5,34℃,rotation rate 120 r/min.Under the best condition,the strain could completely degrade 6 g/L nicotine and degrade 30.22％ of 14 g/L nicotine after cultication for 120 h.It showed that the strain AH14 had a strong ability to degrade nicotine,so it could be applied to degrade high concentration nicotine.
国家科技期刊平台
NSTL
万方数据
