Analysis of Molecular Evolution and Codon Bias of Lipoxygenase (LOX) Gene Family in Tea Tree
Lipoxygenase (LOX,EC 1.13.11.12) is a class of non-heme iron-containing enzyme specifically catalyzing the dioxygenation of polyunsaturated fatty acids.Based on the tea tree genome,this study analyzed and concluded the molecular characteristics,evolution law and codon preference of 12 members in tea tree genome.The results of physiochemical nature detect showed that CsLOX6 gene had the longest open reading frame (ORF) of 2 778 bp,encoding 925 amino acids,and its protein molecular weight was 104.5 kDa.Compared with amino acid sequences of the other species,the phylogenetic tree between tea tree LOX gene family members and 37 LOX genes from 26 species(all belong to dicotyledoneae) were constructed.The results showed that 5 members belonged to 9-LOX subtribe,5 members belonged to 13-LOX Type Ⅰ subclass,and only 2 members belonged to 13-LOX Type Ⅱ] subclass.However,as the member of 13-LOX Type Ⅱ subclass,CsLOX5 lacked of transit peptide in its leader sequence.The result of analyzing the relative synonymous codon usage (RSCU) value showed that 4 codons were high-frequency,and 9 codons were low-frequency.The results of the analysis of codon bias parameters showed that the bias of codon usage of tea tree LOX gene family was weak,and they prefered to use A/T as their third place of codons.In addition,cluster analysis of RSCU value was made with Neighbor-Joinin (NJ) method,and it was found that there were differences in CsLOX10 gene clustering between phylogenetic tree based on coding sequences(CDS) and RSCU value clustering.
国家科技期刊平台
NSTL
万方数据
维普
