Improvement of Heterologous Protein Secretion and Folding Pathways of Pichia pastoris
Most of the active proteins and industrial enzymes commonly used in industry,agriculture and medicine are obtained through heterologous expression systems.Pichia pastoris is one of the excellent hosts for expressing heterologous proteins.This expression system has the advantages including good genetic stability,high protein expression and secretory level,post-translational modification system,and low cost etc.However,excessive aggregation of heterologous proteins usually leads to incorrect fold and ineffective secretion of the target proteins,thus decling protein expression level.This paper reviewed the progress in the study on exogenous protein expression in Pichia pastoris by engineering the secretion and folding pathways such as signal peptide optimization,chaperone optimization and fusion protein expression.
Pichia pastorissecretion and folding pathwayssignal peptidemolecular chaperonesfusion expression
国家科技期刊平台
NSTL
万方数据
维普
