毕赤酵母外源蛋白分泌及折叠途径的改良进展
Improvement of Heterologous Protein Secretion and Folding Pathways of Pichia pastoris
路泽群 1刘宁 2张红莲 3王苑 3黄火清3
作者信息
- 1. 河南科技大学动物科技学院,河南 洛阳 471203;中国农业科学院北京畜牧兽医研究所,北京 100193
- 2. 河南科技大学动物科技学院,河南 洛阳 471203
- 3. 中国农业科学院北京畜牧兽医研究所,北京 100193
- 折叠
摘要
工业、农业和医药等领域常用的活性蛋白和工业酶大多数通过异源表达系统获得.毕赤酵母(Pichia pastoris)是优秀的外源蛋白表达宿主之一,以毕赤酵母为宿主的表达系统具有遗传稳定性好、翻译后修饰、蛋白表达和分泌水平高及生产成本低等优点,但在高效表达过程中外源蛋白过量聚集会导致目标蛋白不能正确折叠和有效分泌,从而影响蛋白表达水平.概述了通过信号肽优化、分子伴侣优化以及融合蛋白表达等分泌及折叠途径的改良,从而促进外源蛋白高效表达的研究进展.
Abstract
Most of the active proteins and industrial enzymes commonly used in industry,agriculture and medicine are obtained through heterologous expression systems.Pichia pastoris is one of the excellent hosts for expressing heterologous proteins.This expression system has the advantages including good genetic stability,high protein expression and secretory level,post-translational modification system,and low cost etc.However,excessive aggregation of heterologous proteins usually leads to incorrect fold and ineffective secretion of the target proteins,thus decling protein expression level.This paper reviewed the progress in the study on exogenous protein expression in Pichia pastoris by engineering the secretion and folding pathways such as signal peptide optimization,chaperone optimization and fusion protein expression.
关键词
毕赤酵母/分泌折叠途径/分泌信号肽/分子伴侣/融合表达Key words
Pichia pastoris/secretion and folding pathways/signal peptide/molecular chaperones/fusion expression引用本文复制引用
基金项目
国家重点研发计划(2021YFC2103002)
财政部和农业农村部现代农业产业技术体系建设项目(CARS-41)
出版年
2024
国家科技期刊平台
NSTL
维普
万方数据