Effects of Phosphorus Recovery on Photosynthetic Pigments and Some Antioxidant Enzymes Activities of Phosphorus Starved Microcystis aeruginosa
In order to explore the effects of phosphorus starvation and phosphorus recovery on the physiological indexes of Microcystis aeruginosa,its cells were treated with phosphorus starvation for 7 d and then phosphorus recovery was carried out.The cell density,the contents of chlorophyll a(chl-a),carotenoids,phycocyanin(PC),allophycocyanin(APC),phycoerythrin(PE),malondialdehyde(MDA),hydrogen peroxide(H2O2)and superoxide dismutase(SOD)activity were determined.The results showed that the density of M.aeruginosa was 2.54×107 cell·mL-1,significantly lower than that of the control group(3.11×107 cell·mL-1).After 144 h of phosphorus recovery,the algae density in the treatment group was 4.05×107 cell·mL-1,significantly lower than that of the control group(4.32×107 cell·mL-1).The contents of chl-a,carotenoids and PC,APC,PE showed a trend of gradual increase,which were 5.96、1.44 μg·mL-1 and 0.031,0.02,0.065 mg·L-1 at 144 h.The contents of MDA,H2O2 and the activity of SOD all increased first and then decreased,and reached the maximum value at 48 h,which increased by 36.2%,47.7%and 51.1%,respectively,compared with the control group.In conclusion,the cell density and the contents of Chla,carotenoid,phycobiliprotein of M.aeruginosa increased after phosphorus recovery,but it was difficult to recover to control level.The changes of MDA,H2O2 contents and SOD activity indicated that M.aeruginosa cells were damaged by oxidation and the cell membrane system was damaged by phosphorus starvation and phosphorus recovery.
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NSTL
万方数据
维普
