Effects of Soil Moisture Content on Microbial Community Diversity and Abundance of Nitrogen Cycling Genes in Central Henan Tobacco-growing Soil
To investigate the dynamic changes of nitrogen mineralization of tobacco-planting soil in central Henan and explore the response characteristics of soil microbial diversity and nitrogen cycle function genes to soil moisture condition,the tobacco-planting soil from Xuchang were cultured under 50%(H-50%),65%(H-65%),80%(H-80%)water conditions to analyze the difference of functional diversity of bacterial and fungal communities.The results showed that the mineralization amount and mineralization rate of soil inorganic nitrogen in H-65%treatment were higher than those in other treatments.Proteobacteria,Actinobacteria,Chloroflexi,Firmicutes,Acidobacteria,Planctomycotes,and Germatimonades were the dominant bacteria at phylum level(relative abundance>3%).The relative abundance of Proteus in H-80%treatment was significantly higher than other treatments,while the relative abundance of Firmicutes was vice versa.The relative abundances of Actinomyces and Curvularia in H-50%treatment were significantly higher than those in other treatments.Ascomycota accounted for more than 90%of the fungal OTUs(operational taxonmic units),and its relative abundance showed an inverted"V"trend with the increasing of soil moisture content.The results of LEfSe(LDA effect size)analysis for bacterial showed that 6 active biomarkers(LDA value>3.5)were detected at the genus level.The bacterial community showed abundant functional diversity,the primary functional layer was more active in metabolism,and the functional gene abundance of the secondary functional layer changed obviously under different water content conditions.The relative abundances of nifK,nifD,nifH genes involved nitrogen fixation process showed H-50%>H-65%>H-80%,and the relative abundances of norB,nirK,nosZ genes involved denitrification process were the highest in H-65%treatment.Collectively,the reasonable regulation of soil moisture content might effectively regulate the dynamic change of soil nitrogen mineralization,the functional diversity of soil microbial community and the change of functional genes relating to nitrogen cycle in central Henan tobacco growing areas.
nitrogen mineralizationsoil moisture contentnitrogen cyclingfunctional genessoil microbial community structure
国家科技期刊平台
NSTL
万方数据
维普
