Development and Effect Evaluation of KASP Markers for Fiber Strength in Gossypium hirsutum L.
Fiber strength(FS)is an important indicator of fiber quality traits,and developing molecular markers associated with target traits would improve the selection accuracy and accelerate the process for breeding.The selected KASP markers were genotyped among 376 varieties(lines),and then the polymorphism and the efficacy for higher strength were analyzed.3 SNP markers showed polymorphic in the population,and the selection rate of FS-15 for high-strength fiber was 63.2%while FS-16 and FS-29 were 60.0%and 56.1%,respectively.Furthermore,the evaluation efficacy of different haplotype combination(Hap1,Hap2,Hap3 and Hap4)suggested that the accessions harboring Hap3(TTA)showed higher fiber strength than those harboring other haplotypes.The selection rate of Hap3 for higher strength was 72.7%.In addition,Hap3 had no adverse effect on fiber length,boll weigh,seed index and lint index.The above results indicated these KASP markers and Hap3 could be used for selection of higher strength materials by providing genotypic information and did not adversely affect the fiber length,boll weight,seed index and lint index.