Isopentenyltransferase(IPT)is the first limiting enzyme for cytokinin biosynthesis,so to analyze the function of the IPT gene family in Brassica rapa L.,a total of 13 BrIPT genes were identified from the genome of Brassica rapa L.by bioinformatics,which were heterogeneously distributed on 7 chromosomes.These BrIPT genes were divided into 4 substrates and each BrIPT gene included 8～10 conserved motifs and 1～2 UTR regions.The promoter regions of BrIPT genes contained numerous responsive elements.The BrIPT genes were regulated by environmental factors,biohormones,defense and stress.The qRT-PCR results showed that the tRNA-IPT genes BrIPT4,BrIPT6 and BrIPT9 were expressed in various parts of Brassica rapa L..The expressions of IPT genes in Brassica rapa L.were higher at the mature stage than those at the seedling stage.The above results provided some basis for subsequent in-depth studies on the physiological and biochemical functions of IPT gene family members.
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