Characteristics of Temporal Expression of Key Genes During Differentiation from Sheep Preadipocyte
To analyze the expression pattern and characteristics of key genes in sheep preadipocytes during adipogenic differentiation,3T3-L1 cell line was selected as reference,the preadipocytes were isolate from the tail adipose tissue of female lambs from Hulun Buir sheep 20-day after birth using tissue digestion method and prepared into primary cell line.The 3T3-L1 cell line and sheep preadipocyte cells were induced to differentiate into mature adipocyte by cocktail method,and the temporal expressions of key genes during differentiation were detected by real-time fluorescence quantitative PCR.The results showed that the adipogenic differentiation of sheep precursor adipocytes took longer,and some cells still had differentiation ability after 16 d of induction.The patterns of temporal expression indicate that the expressions of DLK1,SREBP1,ACC1 and FASN were basically consistence both in the sheep preadipocytes and 3T3-L1 cell lines,the expression trends of ZFP423,ADIPOQ and C/EBPα were highly similar,however,the genes of PPARγ,FABP4,LPL and HSL were different.Compared with 3T3-L1 cell line,the relative expression levels of ZFP423,ADIPOQ,C/EBPα and FABP4 in sheep preadipocytes still remained at a high level in the middle and late stages of induced differentiation,and the relative expressions of PPARγ,LPL and HSL showed a continuous increase trend,which illustrated that sheep preadipocytes had its own characteristics in adipogenic differentiation.It was speculated that the cells from the sheep adipocytes synthesized triglycerides and then deposited in mature adipocytes through ingesting fatty acids from the external environment after 16 d of induction according to the expression of related genes.It indicated that sheep adipose cells had strong ability to absorb external fatty acids,which might be one of reasons for the excessive deposition of subcutaneous fat in native sheep.So the sheep adipocyte was more suitable as a cell model in research of sheep fat metabolism,which provided reliable experimental model for research of sheep fat metabolism.
万方数据
维普
