In order to explore the immune effect of inactivated vaccine of Nocardia seriolae on grouper,the immune protection of the vaccine on grouper was analyzed by the expression of immune gene.In this study,formalin-inactivated vaccine was prepared by this bacteria,and the pearl gentian grouper was immunized by intraperitoneal injection.The expression changes of TLR2,MyD88,TNF-α and IL-1β immune gene in the kidney,liver and spleen of the fish were detected by fluorescence quantitative method.The results showed that by injecting inactivated vaccine,the expressions of TNF-α and IL-1β genes were higher in kidney tissues,MyD88 gene's expression was higher in spleen tissues,and TLR2 gene's expression was higher in liver tissues.Except for a trend of decreasing relative expression of TNF-α gene in the tissue spleen,and the other genes showed up-regulation in all organs compared with that in blank group.Above results provided a theoretical basis for exploring the interaction between pearl gentian grouper and Nocardia seriolae,and laid a foundation for the development and application of fishery vaccines.
维普
万方数据