Identification of Pathogen Causing Bacterial Fruit Blotch of Melon in Hainan
To identify the pathogen which seriously caused fruit blotch disease in melo,the melon fruit samples suspected bacterial fruit blotch disease were collected from Dongfang city,Hainan province.Pathogen was isolated and purified from samples,and the pathogenicity of bacterium strains was confirmed according to Koch's postulate.The identification of the pathogenic strains was carried out according to the colony morphology,physiological and biochemical analysis and 16S rDNA sequences analysis.The results showed that the isolated bacterium with similar cultural characteristics was obtained from all diseased samples,which the separation rate of bacteria was 100％.All of tested strains showed pathogenic to melon fruits.Strains were identified as Gram-negative bacterium,which colony morphology,physiology and biochemistry shared similarly with that of Acidovorax citrull.BLAST analysis showed that the 16S rDNA sequences had 100％homology with that of A.citrulli,and the phylogenetic analysis showed the pathogen was A.citrulli.Above results determined the pathogen of melon fruit blotch,which provided a basis for effective prevention and control of this disease.
melonbacterial fruit blotchpathogen identificationAcidovorax citrulliDongfang city in Hainan province
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