Heat Stress Response and Comprehensive Evaluation of Heat Resistance of Lentinula edodes Mycelia
In order to study the response of mycelia of different Lentinula edodes strains to heat stress,comprehensively evaluate their heat resistance and screen out key indicators,9 Lentinula edodes strains were used as experimental materials,and 25℃ normal temperature culture(CK)and 37℃ heat stress treatment were carried out during the mycelia growth period.The growth rate,dry weight,thiobarbituric acid reactive substances(TBARS),protein carbonyl(PCO),superoxide dismutase(SOD),catalase(CAT),peroxidase(POD),laccase,carboxymethyl cellulase,hemicellulase and amylase activities of mycelia under different treatments were determined.The comprehensive evaluation of heat resistance and screening of key indicators were carried out by correlation analysis,principal component analysis,membership function and cluster analysis.The results showed that the growth rate and dry weight of mycelia after heat stress were significantly lower than those of CK,while the contents of TBARS,PCO and the activities of SOD,CAT,laccase,carboxymethyl cellulase,hemicellulose and amylase were extremely significantly higher than those of CK,and the activity of POD was significantly higher than that of CK.Among 9 strains,Xinqiu 7 had the biggest variation range on the growth rate,the activities of laccase,hemicellulose,amylase,and the TBARS content,which were 66.55%,82.42%,46.03%,75.27%and 23.64%,respectively.The dry weight of 109 decreased the most,which was 77.81%.The PCO content of 808 increased the most,which was 43.77%.The activities of SOD and POD of 212 were 1.64 and 4.48 times of CK,respectively.The CAT of Jiuxiangqiu 7 increased by 49.90%.The carboxymethyl cellulase activity of 1513 decreased by 60.87%.Correlation analysis showed that there was a significant positive correlation between SOD and POD activity,and POD activity was significantly negatively correlated with TBARS content.Based on the comprehensive evaluation D value of heat resistance,9 strains were classified into 3 types.TypeⅠ included 212,Jiuxiangqiu 7,238 and 0912;type Ⅱ included 9608,808,1513 and 109;type Ⅲ only included Xinqiu 7;and the heat resistance of 3 types showed Ⅰ>Ⅱ>Ⅲ.The best regression model was established by stepwise regression analysis.The growth rate of mycelia,TBARS content,SOD and POD activity were selected as the key identification indexes,which could quickly identify the heat resistance of mycelia.Above results provided a theoretical basis for the breeding of heat-resistant core germplasm of Lentinula edodes in southern Xinjiang.
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