Inhibition Mechanism of Water Extract from Spent Mushroom Substrate against Phytophthora infestans in potatoes
Mushroom substrate is the residue of mushroom production,which is rich in inorganic salts and organic matter.The water extract from spent mushroom substrate(WESMS)of Lentinula edodes was prepared by hot water extraction,the ultraviolet(UV)absorption,surface charge and particle size distribution were characterized using an UV spectrophotometer,Zeta potential and particle size analyzer.The inhibition rate of WESMS against Phytophthora infestans was calculated by plate penetration and cross diameter measurement.Further,both optical microscopy and scanning electron microscopy were used to observe the damage caused by WESMS to the morphology of the pathogen cells.Subsequently,the impact of WESMS on the DNA of Phytophthora infestans was analyzed by agarose gel electrophoresis.The results showed that WESMS could absorb UV rays at A(UVA,315～400 nm),B(UVB,280～315 nm)and C(UVC,100～280 nm)bands.Moreover,the particle size distribution in water was 3 649.27 nm.The antibacterial experiment showed that WESMS had an inhibitory effect on the growth of pathogens.The inhibitory effect became more obvious with the increase of the volume percentage concentration of WESMS.The inhibition rate of WESMS against pathogens was nearly 100%at a high volume concentration of 6.25%.The mycelia of pathogenic cells treated with WESMS exhibited obvious distortion,flatness and wrinkles compared with the control group,which indicated that WESMS could cause severe damage to cells.In addition,the DNA band brightness of the treatment group was darker than that of the control group,and the degree of DNA damage was positively related to the volume percentage concentration of WESMS.Above results provided scientific basis and technical support for the utilization of spent mushroom substrate and its use in the effective control of potato late blight.
spent mushroom substrate of Lentinula edodespotato late blightPhytophthora infestansantimicrobial mechanism
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