Bactericidal Mechanism of Antimicrobial Peptide PAJE Against Staphylococcus aureus and SCVs
Staphylococcus aureus(S.aureus)and the small colony variants(SCVs)infection are considered as main issues affect livestock and poultry health.However,there are still many challenges in persistent and recurrent infections,and even drug resistance for treatment of traditional antibiotics.The antimicrobial peptide PAJE has the broad-spectrum antibacterial activity.To study the mechanisms of peptide PAJE against S.aureus and SCVs,the antibacterial activity,stability and biological characteristics were determined.The results indicated that the antibacterial peptide PAJE had a penetration ability to destruct bacterial cell wall,even permeated 54.7%and 62.9%cell membrane.Additionally,the peptide PAJE could also up-regulate the Agr regulatory system genes,down-regulate the SigB regulatory system and biofilm forming related genes,which greatly inhibited biofilm formation.In addition,higher temperature and pH stability made PAJE better processing,storage,and environmental tolerance.In conclusion,the antimicrobial peptide PAJE could be a potential candidate peptide for the treatment of S.aureus,SCVs and their biofilm infections,which provided theoretical support for the development of reduce and replace antibiotics products.
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