小麦TaCOBL-5基因克隆及表达分析
Cloning and Expression Analysis of Wheat TaCOBL-5 Genes
鲍新跃 1陈红敏 2王伟伟 3唐益苗 3房兆峰 3马锦绣 3汪德州 3左静红 3姚占军4
作者信息
- 1. 河北农业大学农学院,河北 保定 071033;北京市农林科学院杂交小麦研究所,北京 100097
- 2. 濮阳市现代农业发展中心,河南 濮阳 457177
- 3. 北京市农林科学院杂交小麦研究所,北京 100097
- 4. 河北农业大学农学院,河北 保定 071033
- 折叠
摘要
小麦产量关系我国粮食安全,干旱、低温、盐害和高温等非生物胁迫严重制约小麦产量增长.前期转录组分析发现小麦TaCOBL-5D在多种非生物胁迫下差异表达.克隆并获取TaCOBL-5D及其同源基因TaCOBL-5A、TaCOBL-5B,并对其生物信息学特性及表达模式进行了分析.结果显示,TaCOBL-5与其他物种的COBL基因在基因结构、蛋白三级结构、保守结构域以及启动子调控元件方面表现出明显的保守性.TaCOBL-5在根部表达量最高,并对各种非生物胁迫响应不同,尤其是在干旱胁迫下调控显著,表明其在干旱胁迫中的重要性,同时对低温、高温和盐胁迫也有不同响应.此外,TaCOBL-5D基因在不同干旱抗性及高温抗性材料中表达量差异显著,进一步暗示其在逆境胁迫中具有重要作用.这些研究结果有助于理解COBL基因在小麦中的功能,同时为小麦抗逆育种提供科学支持.
Abstract
Wheat yield directly impacts food security in China,but various abiotic stresses such as drought,low temperature,salt and high temperature severely constrain wheat production.In previous analysis,transcriptomic data revealed differential expression of wheat TaCOBL-5D under multiple abiotic stresses.TaCOBL-5D and its homologous genes TaCOBL-5A and TaCOBL-5B were cloned and obtained,and the comprehensive bioinformatics and expression pattern were analyzed.The results showed that TaCOBL-5 genes exhibited significant conservation in gene structure,protein tertiary structure,conserved domains,and promoter regulatory elements with COBL genes from other species.TaCOBL-5 genes exhibited the highest expression level in roots and responded differently to various abiotic stresses,especially significantly down-regulated under drought stress,indicating its importance under drought stress;it also showed different responses to low temperature,high temperature,and salt stress.In addition,the expression level of the TaCOBL-5D gene varied significantly among different drought-resistant and high-temperature-resistant materials,further suggesting its important role in stress resistance.These findings laid the foundation for further understanding of the function of COBL genes in wheat and provided scientific support for wheat breeding efforts aimed at enhancing stress tolerance.
关键词
小麦/COBL基因/生物信息学分析/非生物胁迫/表达模式Key words
wheat/COBL genes/bioinformatics analysis/abiotic stress/expression pattern引用本文复制引用
基金项目
北京市农林科学院协同创新中心项目(KJCX201907-2)
北京市农林科学院青年科研基金(QNJJ202316)
出版年
2024
国家科技期刊平台
NSTL
万方数据