Cloning and Expression Analysis of Wheat TaCOBL-5 Genes
Wheat yield directly impacts food security in China,but various abiotic stresses such as drought,low temperature,salt and high temperature severely constrain wheat production.In previous analysis,transcriptomic data revealed differential expression of wheat TaCOBL-5D under multiple abiotic stresses.TaCOBL-5D and its homologous genes TaCOBL-5A and TaCOBL-5B were cloned and obtained,and the comprehensive bioinformatics and expression pattern were analyzed.The results showed that TaCOBL-5 genes exhibited significant conservation in gene structure,protein tertiary structure,conserved domains,and promoter regulatory elements with COBL genes from other species.TaCOBL-5 genes exhibited the highest expression level in roots and responded differently to various abiotic stresses,especially significantly down-regulated under drought stress,indicating its importance under drought stress;it also showed different responses to low temperature,high temperature,and salt stress.In addition,the expression level of the TaCOBL-5D gene varied significantly among different drought-resistant and high-temperature-resistant materials,further suggesting its important role in stress resistance.These findings laid the foundation for further understanding of the function of COBL genes in wheat and provided scientific support for wheat breeding efforts aimed at enhancing stress tolerance.
国家科技期刊平台
NSTL
万方数据
