Effect of Microbicides on Main Diseases and Soil Microbial Communities of Tomatoes in Facilities
To investigate the effects of microbial agent on tomato yield,disease incidence,soil physicochemical properties and microbial community structure,no microbial agent was as control(CK),and Bacillus subtilis was applied with 1(T1),2(T2)and 3(T3)times in 1～3 months after tomato transplanting.The incidence and yield of tomato plants and soil physicochemical properties and microbial community structure under different treatments were determined.The results showed that,compared with CK,the yield of T1,T2 and T3 treatments significantly increased by 16.60%,39.58%and 29.43%,and the incidence of gray mold,leaf mold and late blight significantly reduced.Alpha diversity indices showed that Chao and Shannon indices of soil bacteria significantly increased in T1,T2 and T3 treatments;Chao and Shannon indices of soil fungi significantly decreased in T1,T2 and T3 treatments,which indicated that the addition of microbial agent increased the diversity and richness of bacterial communities and decreased the diversity and richness of fungal communities.In addition,the relative abundance of Actinobacteria,Bacteroidetes,Gemmatimonadetes,Firmicutes,Patescibacteria in bacterial and Mortierellomycota,Basidiomycota,Chytridiomycota,Olpidiomycota and Calcarisporiellomycota in fungi were increased with the increase of the number of microbial agents,while the relative abundance of Chloroflexi,Acidobacteria in bacterial and Ascomycota in fungi were decreased.Redundancy analysis(RDA)showed that the soil organic matter,total nitrogen,alkaline nitrogen,effective phosphorus,and fast-acting potassium were more correlated with bacterial community structure,and soil organic matter content,total potassium,effective phosphorus,fast-acting potassium,pH,and electrical conductivity were more correlated with fungal communities.In conclusion,the applications of microbial agent could significantly reduce the incidence of diseases,increase the yield of tomatoes in facilities,improve the microbial community structure in the soil,and regulate the soil microbial community toward healthy direction.
tomatomicrobial agentmicrobial communitiesphysical and chemical properties of soil
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