Research Status and Development Trend of Rapid Detection of Agglutinin in Concanavalin A
Concanavalin A(Con A)is mainly found in jack bean,and it is a plant lectin that can specifically bind to polysaccharides,glycoproteins and other substances.Con A has anti-nutritional and anti-pest effects,which is an important part of plant self-protection,and also the main cause of food poisoning and allergic reactions caused by eating legumes.Therefore,the detection of Con A is of great significance for legume vegetable breeding and food safety assessment.The research progress of novel rapid detection technologies for Con A was introduced in detail,including nanomaterial based biosensor,immune sensing technology,sugar sensing technology and aptamer sensing technology.Biosensors based on nanomaterials had high accuracy but lack biological specificity;the immune sensing technology had improved the detection speed of the traditional immune method but still had the problem of insufficient signal repeatability;sugar sensing technology had greatly improved the specificity and detection accuracy of hemagglutination method,but it could not distinguish lectins with the same sugar specificity.Aptamers based detection technology had excellent detection accuracy,specificity and repeatability,and unnatural aptamers could be obtained through chemical modification to further enhance the specificity,which should be the development trend of rapid detection of Con A in the future.
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