Optimization of Chinese Chestnut Yellow Crinkle Detection Method and Resistance Identification of 10 Chestnut Varieties
In order to optimize the detection method of Chinese chestnut yellow crinkle and clarify the resistance of 10 chestnut varieties,the resistance evaluation was carried out by optimizing the content of nested PCR primers and annealing temperature,combined with phytoplasma detection rate and field disease index.The results showed that the detection rate of chestnut phytoplasma was the most stable when the primer addition amount was 1.0 µL and the annealing temperature was 54℃.After 400 d of grafting,phytoplasmas were detected in all the tested chestnut varieties.Among them,the detection rate of phytoplasma in the leaves of'Yanshan Zaofeng Yabian'was the highest and the incidence was the most serious,while the detection rate of phytoplasma in the leaves of'Yanli'was the lowest and the incidence was the lightest.The correlation analysis showed that the detection rate of phytoplasma was significantly positively correlated with the field disease index,the correlation coefficient was 0.907,and the linear regression equation was Y=0.276X+1.610.According to 2 evaluation methods,the tested chestnut varieties were divided into high resistance,medium resistance and high sensitivity.Among them,'Yanqiu''Yanzi''Yanlong'and'Yanli'were high resistance varieties,and'Yanshan Zaofeng Yabian'was high sensitivity varieties.The resistance difference of 10 chestnut varieties to Chinese chestnut yellow crinkle was preliminarily clarified,which provided basic data for the selection of resistant varieties.
国家科技期刊平台
NSTL
万方数据
