为深入探索老山芹种子休眠机理，以老山芹层积前后种子为研究对象，采用气相色谱-飞行时间质谱联用技术(gas chromatography-time of flight mass spectrometry，GC-TOF/MS)对其非层积种子(non-lamellarized seeds，NS)和层积后达到发芽状态种子(germination seeds，GS)的差异代谢物进行分析。结果表明，共检测到995种代谢产物，NS和GS的代谢物存在明显分离，差异代谢物有126种，其中极显著差异代谢物75种，包括35种上调，40种下调。对极显著差异代谢物所在代谢通路分析表明，涉及10条关键代谢通路。和弦图分析表明，差异代谢物中脂质类化合物(含类脂分子)与苯类化合物呈极显著正相关;苯丙烷类化合物(含聚酮类化合物)与有机氧类化合物呈极显著负相关。以上研究结果为探索老山芹种子成熟后萌发提供了代谢组学理论基础，为更好地研究老山芹种子后成熟及休眠机制提供了依据和支撑。
Analysis of Differential Seed Metabolites Before and After Stratification of Heracleum moellendorffii Hance
In order to deeply explore the seed dormancy mechanism of Heracleum moellendorffii Hance,the different metabolites of non-lamellarized seeds(NS)and germination seeds after lamellarization(GS)were analyzed by GC-TOF/MS(gas chromatography-time of flight mass spectrometry).The results showed that a total of 995 metabolites were detected,and there was a clear separation between NS and GS.There were 126 differential metabolites,among which 75 were extremely significant differential metabolites including 35 up-regulated and 40 down-regulated expressions.The metabolic pathways of the extremely significant differential metabolites were analysed and 10 key metabolic pathways were identified.The results of the chord diagram analysis showed that the lipids(including lipid-like molecules)was extremely significantly positive correlaed with benzenoids,while the benzenepropanoids(including polyketides)was extremely significantly negative correlation with organoxides.Above results provided theoretical basis of metabolomics for exploring the post-maturation germination of Heracleum moellendorffii Hance seeds,and researching the post-maturation and dormancy mechanisms of Heracleum moellendorffii Hance seeds.
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