为探索长期连作状态下烟草根系分泌物内含有的化感物质组成成分，设置石英砂栽培和连作土壤栽培2种烟草培养处理，对2种烟草不同生长时期的根系分泌物进行提取，利用超高效液相色谱串联质谱(ultra-high performance liquid chromatography tandem mass spectrometry，UHPLC-MS/MS)对提取的根系分泌物进行分析，通过数据库比对、差异分析和时间聚类分析等方法筛选连作烟草根系分泌物中潜在的化感物质。结果表明，共筛选出11类潜在化感物质，分别为生物碱、萜类、胺类、有机酸、苯酸及其衍生物、脂肪酸、酚类、氨基酸和多肽、香豆素、肉桂酸及其衍生物和其他。生物碱、萜类、胺类和有机酸4类化合物相对含量占所有化合物的73。3%。L-烟碱、熊果酸、β-氨基丙腈、5-羟基吲哚乙酸、早熟素Ⅱ、单乙基己基邻苯二甲酸、己内酰胺、姜酚、L-乳酸、邻苯二甲酸二乙酯和2，3-二氢-3，5-二羟基-2-氧代-3-吲哚乙酸甲酯是连作烟草根系分泌物中相对含量较高的潜在化感物质。研究结果为烟草根系化感物质的化感作用研究及缓解烟草连作障碍方法研究提供理论基础。
Identification of Continuous Cropping Tobacco Root Exudates and Screening of Potential Allelopathic Substances
To explore the components of allelopathic substances contained in tobacco root exudates under long-term continuous cropping,quartz sand cultivation and continuous soil cultivation 2 tobacco culture experiments were set up,root exudates of non-continuous cropping and continuous cropping tobacco at different growth stages were extracted,and the extracted root exudates were identified and analyzed by ultra-high performance liquid chromatography tandem mass spectrometry(UHPLC-MS/MS),then potential allelopathic substances in the root exudates of continuous cropping tobacco were screened by database comparison,difference analysis and time cluster analysis.The results showed that a total of 11 potential allelopathic substances were screened,namely alkaloids,terpenes,amines,organic acids,benzic acids and derivatives,fatty acids,phenols,amino acids and peptides,coumarins,cinnamic acid and derivatives and others.The relative content of alkaloids,terpenes,amines and organic acids accounted for 73.3%of all compounds.L-nicotine,ursolic acid,β-aminopropionitrile,5-hydroxyindoleacetic acid,precocene Ⅱ,monoethylhexylphthalic acid,caprolactam,gingerol,L-lactic acid,diethyl phthalate,and methyl 2,3-dihydro-3,5-dihydroxy-2-oxo-3-indoleacetate were potential allelopathic substances with relatively high levels in continuous tobacco root exudates.This study provided a theoretical basis for the study of allelopathic substances in tobacco roots and the research on methods to alleviate tobacco continuous cropping disorders.
国家科技期刊平台
NSTL
万方数据
