Screening,Identification and Biocontrol of Bacteria Degrading Ginseng Phenolic Acid Autotoxic Substances
with the increase of ginseng planting years,the accumulation of autotoxic substances in the soil results in the occurrence of continuous cropping obstacles,which significantly impeding the robust advancement of ginseng cultivation.The biodegradation of autotoxic compounds in the soil is as an effective strategy to mitigate the continuous cropping obstacles.The bacteria degrading phenolic acid autotoxic substances were isolated and identified from the ginseng rhizosphere soil.Using 16S rRNA gene sequencing along with physiological and biochemical experiments,the degradation strains were classified and identified.The degradation rate was assessed through UV spectrophotometry,while the culture conditions were refined through a single-factor experiment.The biocontrol of degradation strains on ginseng seeds with phenolic acid stress was investigated.The results showed that 10 strains of bacteria capable of degrading autotoxic substances were isolated from the inter-root soil of ginseng,predominantly belonging to the genus Pseudomonas.Preliminary degradation experiments indicated that strain S1 exhibited the highest degradation rate on salicylic acid,reaching 65.32%,and was identified as Burkholderia.The results of single-factor experiment indicated that the degradation rate of strain S1 was 88.58%with calcium nitrate as the nitrogen source,cultivation temperature of 30℃,and 500 mg·L-1 autotoxic substances,which was higher than before optimization.Strain S1 could alleviate the inhibitory impact of salicylic acid on the growth of ginseng seeds,achieving a growth promotion efficiency of 12.56%.In conclusion,the Burkholderia S1 strain isolated from soil exhibited a commendable biocontrol effect,which had promising application value for mitigating continuous cropping obstacles.
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