Effects of Combined Application of Biochar and Organic Fertilizer on Soil Physicochemical Properties and Microbial Community Characteristics in Apple Recropping Field
In order to investigate the effect of biomass charcoal and organic fertilizer on the improvement of continuous apple nursery land,the effects of different ratios of biomass charcoal and organic fertilizer on the physicochemical properties and microbial community structure of apple inter-root soils were investigated using high-throughput sequencing technology combined with soil physicochemical property analysis,using apple nursery land with 5 years of continuous cultivation.The results showed that the nutrient content of the soil increased significantly,and the pH and conductivity of soil increased significantly after the application of biomass charcoal and organic fertilizer.The quick-acting nutrient content of soil with 600 kg·hm-2 of biomass charcoal increased significantly,and the contents of soil total nitrogen,alkaline nitrogen,available phosphorus,available potassium and organic carbon increased by 15.06%,27.03%,61.32%,51.55%and 20.18%,respectively.In addition,biomass charcoal and organic fertilizer treatments increased the diversity and abundance of bacterial communities in soil and decreased the diversity and abundance of fungal communities,and the abundance of beneficial bacterial genera in soil were all significantly increased.The correlation analysis results showed that the soil organic carbon,available potassium and alkaline nitrogen had significant effects on bacterial community characteristics in soil,while fungal community characteristics were mainly affected by the soil total nitrogen,organic carbon and available potassium.In conclusion,the combination of biomass charcoal and organic fertilizer had a certain mitigating effect on the continuous crop barrier in apple nursery land,which improved the microenvironment of the soil and enhanced the soil quality.
applecontinuous cropping obstaclebiocharsoil physical and chemical propertiescharacteristics of microbial community