Discovering of QTLs and Candidate Genes Related to Rice Heading Period Traits Based on BSA-seq
As one of the important traits of rice,rice heading date is closely related not only to the rice growth period but also to rice yield,quality and stress resistance,which determines the planting area and seasonal adaptability of rice varieties.Therefore,the location of rice heading date related genes plays an important role in rice production.In this study,HQ20,Jin 23B and their F4 population constructed were used as the materials.According to the heading stage of the rice in the population,it was divided into 3 levels including early,middle and green plant(not heading),and 3 mixed pools of early,middle and green were constructed.The bulked-segregant analysis sequencing(BSA-seq)method was used to discover rice heading period genes.The results showed that the comparison rate between BSA-seq results and the reference genome was over 94％,and the 4x genome coverage rate was over 80％.Based on this,when the confidence level was 0.95,by SNP-index algorithm 17 SNPs located on 4 chromosomes(6,7,8 and 9)were obtained which had consistent association intervals with indel.Further analysis of candidate genes in the region found that 61 genes had non-synonymous mutations.GO and KEGG enrichment analysis showed that pathways closely related to rice heading stage included UDP-glycosyltransferase activity,positive regulation of cell cycle G2/M phase transition,and phytohormone signal transduction.Finally,combining haplotype analysis in the 3K rice database and published expression data,3 key candidate genes for heading stage were discovered including LOC_Os07g22720,LOC_Os07g23740 and LOC_Os08g07200.The above results laid the foundation for genetic improvement of rice heading period traits and analysis of genetic basis.
万方数据
维普
