Bioinformatics Analysis of Porcine CREBRF Gene and Its Expression Pattern
CREBRF gene plays an important regulatory role in the reproductive process of female animals.In order to deeply understand the characteristics and functions of CREBRF gene in pigs,bioinformatic analysis of the CREBRF gene was performed,and the expression pattern of CREBRF in ovarian tissues at different stages of Bama pigs was analyzed by RT-qPCR.The results showed that 20 mutations of CREBRF gene were identified in the genomes of 5 pig breeds,7 of which were synonymous mutations and 13 were missense mutations.The CDS region of the porcine CREBRF gene was 1 920 bp in length,encoding a total of 639 amino acids,with a high instability coefficient,a hydrophilic protein,85 high-confidence phosphorylation sites and 4 glycosylation sites,and the mRNA of CREBRF had 20 high-confidence m6A methylation modification sites.CREBRF proteins were more conserved in pig,human and mouse,and the secondary structures of pig and human CREBRF proteins were more similar.RT-qPCR results showed that the expression of the CREBRF gene in pig ovaries was higher at 0 and 28 d after birth,and with the growth and development of pigs,the expression decreased significantly at 180 d after birth,and the expression of CREBRF increased again at 38 and 80 d after gestation in sows.By comparing the base mutations in the CDS region of CREBRF gene in different breeds of pigs and analysing the physicochemical properties and expression pattern of the gene,CREBRF gene was highly expressed in the ovarian tissues of pigs at the fetal stage,early birth and gestation,and it was speculated that CREBRF plays an important role in the reproductive process of sows,which provided a reference for the mining of genes for high porcine fertility and the development of molecular markers.
万方数据
维普
