Effects of Microorganisms from Different Sources on the Composting Process of Grape Branches and Pig Manure
In order to explore the influence of microorganisms from different sources on the composting process of grape branch and pig manure,3 treatments were set including no external microorganisms(CK),inoculated biogas slurry(T1),and inoculated microbial inoculum(T2).The temperature,moisture content,pH,electrical conductivity(EC),total nitrogen(TN),total phosphorus(TP),total potassium(TK),seed germination index(GI),bacterial and fungal communities were analyzed during the composting process to determine the key microbial in the composting process.The results showed that the temperature of T1 was higher than that of T2 treatment in the early stage of composting with over 45 ℃ at the 3rd day,and the maximum temperature all reached 60 ℃ in 3 treatments.The changes of moisture,pH,EC and nutrient content of compost in each treatment were consistent,and the contents of TN,TP and TK were 3.43％～3.59％,1.46％～1.56％and 1.87％～2.02％,respectively.The moisture content,pH and EC of compost in T2 were higher than those in other treatments after composted.The germination indexes of CK and T1 treatment exceeded 70％on the 30th day,and reached the organic fertilizer standard faster than T2 treatment.The results of microbial community analysis showed that the relative abundance of Proteobacteria and Firmicutes exceeded 75％in the early stage,Firmicutes was the dominant phylum in the middle stage,and the relative abundances of Bacteroides and Deinococcus-Thermus increased in the middle and final stages,and their final proportions exceeded 20％.There were significant correlations between Firmicutes,Bacteroides,Deinococcus-Thermus and indicators of composting.The analysis of contribution degree showed that the total contribution degree of microbial community to composting was 33.90％,and the contribution degree of Gemmatimonadetes was 80.80％.Above results provided a reference for further research and screening of special antioxidant resistant microorganism inoculants.
grape branchesmicroorganism inoculantsbiogas slurrycompostmicrobial community
万方数据
维普
