Analysis of High-efficiency Transcriptome of Nitrogen in Millet Seedlings and Gene Mining
Nitrogen use efficiency is an important factor affecting the yield and quality of millet.It is of great significance to screen millet varieties with high nitrogen efficiency and explore the important genes controlling nitrogen use efficiency and their mechanism for improving nitrogen use efficiency of millet,reducing fertilizer use,protecting the environment and ensuring food security.By comparing phenotypic traits,nitrogen absorption capacity,it showed that nitrogen-efficient varieties had high ammonium nitrogen and nitrate nitrogen absorption capacity than nitrogen inefficient varieties.3 nitrogen level treatments were set,0,3 and 6 mmol·L-1 and after the rice seedlings at two-leaf one-stage were treated for 7 d.Taking 0 mmol·L-1 nitrogen treatment as control,a total of 16 000 differential expression genes(DEGs)were obtained by RNA-seq.Through GO and KEGG enrichment analysis,it was found that amino acid synthesis and metabolism,defense-related metabolism,acetaldehyde acid and dicarboxylic acid metabolism,and carbon fixation of photosynthetic organisms,pyruvate metabolism,porphyrin and chlorophyll metabolism,MAPK signaling pathway-plant,amino sugar and nucleotide sugar metabolism played important roles in regulating the biological reaction of nitrogen metabolism.3 348 transcription factors were predicted,which belonged to 46 families,among which the largest number of predicted transcription factors is the MYB-related family(189),followed by the bHLH family(182),the WRKY family(158),the MYB family(156)and HB-Other families(153).Through WGCNA analysis,the top 30 genes with KME values were screened,and 7 of them were annotated to genetic information.RT-qPCR results showed that the expression trends of Seita.9G488700,Seita.2G368800 and Seita.5G063300 were basically same as RNA-seq results,which mainly regulated the transportation and metabolism of glucose transporters and carbohydrates..
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