Difference Analysis of Different Maturity of Upper Tobacco Leaves Based on Non-targeted Metabolomics
To explore the effects of different harvest maturity on internal quality of upper leaves and metabolomics of tobacco,Yunyan 87 was used as the experimental material,and the leaves were sampled with SPAD value of 30～35(BM1),25～30(BM2),20～25(BM3),15～20(BM4).The effects of different harvest maturity on the agronomic traits,chemical composition coordination,sensory quality and metabolomics of upper leaves were analyzed.The results showed that the upper leaves of BM3 treatment had better coordination of chemical components and the highest sensory evaluation score.A total of 230 differential metabolites were screened from 4 treatments,mainly including organic acids,alkaloids,sugars,amino acids,alcohols and nucleosides.Bioinformatics analysis showed that L-tryptophan,chlorogenic acid,palmitoleic acid,concanavalin,creatine,sorbitol,trigonelline,α-linolenic acid,glyceric acid,traumatic acid,choline,matairesinol,deoxyinosine,rutin,L-phenylalanine,guanine,L-proline,L-methionine,glucaric acid,nicotine,oleic acid,L-serine,flavic acid,L-glutamic acid,syringic acid,eugenol and tryptophan might be the key metabolites during the maturation of upper tobacco leaves.Differential metabolites were significantly enriched in pathways such as amino acid metabolism,nucleotide metabolism,carbohydrate metabolism,vitamin metabolism and lipid metabolism.Among them,glycine,serine and threonine metabolism,phenylpropanoid biosynthesis,alanine,aspartic acid and glutamic acid metabolism pathways played an important regulatory role in the formation of oil and aroma substances during tobacco leaf maturation.
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