首页|小粒咖啡叶绿素合成基因CaPOR的全基因组鉴定及表达分析

小粒咖啡叶绿素合成基因CaPOR的全基因组鉴定及表达分析

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  • 国家科技期刊平台
  • NETL
  • NSTL
  • 万方数据
叶绿素是植物进行光合作用、积累有机物的重要物质,其中POR(pro tochlorophyllide oxidoreductase)负责催化光合体中的光合色素前体原叶绿素酸转化为叶绿素a,是光诱导被子植物转绿的关键酶,对光合作用至关重要.为研究小粒咖啡叶片叶绿素合成POR基因,通过基因家族分析和转录组测序分析,筛选和鉴定小粒咖啡POR基因,并进一步利用实时荧光定量分析关键基因的表达.结果表明,CaPOR基因家族成员蛋白质的理化性质相似,所含保守基序大致相同,在启动子区域主要以光响应元件和生长发育类元件为主.结合染色体定位、共线性分析以及密码子偏好分析发现,CaPOR基因在小粒咖啡中已形成基因簇,且出现复制加快的现象,这一现象是由碱基突变以及自然选择共同影响所致.对筛选出的7个CaPOR基因的表达量进行分析发现,在小粒咖啡叶片的生长发育过程中,7个CaPOR基因的表达量均为成熟叶片显著高于嫩叶,与小粒咖啡叶片生长发育过程中叶绿素合成变化正相关.以上研究结果为进一步揭示小粒咖啡叶片叶绿素合成以及POR基因的功能研究提供参考.
Genome-wide Identification and Expression Analysis of Key Chlorophyll Synthesis Related Gene CaPOR in Coffea arabica
Chlorophyll plays a pivotal role in the process of photosynthesis and the accumulation of organic substances in plants.Pro tochlorophyllide oxidoreductase(POR)is an enzyme that catalyzes the conversion of prochlorophyllide,the precursor of chlorophyll,into chlorophyll a in the chloroplast.It is a critical enzyme for the light-induced greening of angiosperms and is indispensable for photosynthesis.To explore the genes involved in chlorophyll synthesis in Coffea arabica leaves,the transcriptome sequencing analysis was used to identify the chlorophyll synthesis-related genes in C.arabica.Furthermore,the real-time fluorescence quantitative analysis was used to examine the expression of key genes.The results showed that CaPOR gene family members shared similar physicochemical properties and conserved motifs.The promoter regions were primarily composed of light response elements and growth and development elements.Through a combination of chromosome localization,collinearity analysis,and codon usage bias analysis,CaPOR gene had formed a gene cluster in C.arabica,and there was an acceleration of gene duplication,which might be driven by the base mutation and natural selection.The expression levels of 7 selected CaPOR genes were markedly higher in mature leaves than those in young leaves,which was positively correlated with the change in chlorophyll synthesis during the growth and development of C.arabica leaves.Above results provided reference for the chlorophyll synthesis in C.arabica leaves and the functional exploration of POR genes.

Coffea arabicachlorophyllbioinformatic analysisexpression analysis

张振伟、董相书、杨婧、李学俊、杞美军、蒋快乐、杨永林、王步天、施学东、邱俊超、陈治华、葛宇

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云南农业大学热带作物学院,云南 普洱 665099

云南大学资源植物研究院,昆明 650504

小粒咖啡 叶绿素 生物信息学分析 表达分析

云南省创新引导与科技型企业培育计划乡村振兴科技专项云南省"兴滇英才支持计划"青年人才专项云南省基础研究计划面上项目云南省重点研发计划项目云南省专家基层科研工作站项目云南农业大学本科实践教学、劳动教育和耕读教育教学改革研究与实践项目

202304BI090006XDYC-QNRC-2022-0711202301AT070493202303AP1400102021RYZJGZZ0022024SJJX306

2024

10.13304/j.nykjdb.2024.0365

中国农业科技导报
中国农村技术开发中心

中国农业科技导报

CSTPCD北大核心
影响因子：1.252
ISSN：1008-0864
年,卷(期)：2024.26(10)