首页|哈茨木霉菌对烟草的促生及其黑胫病的诱导抗性评价

哈茨木霉菌对烟草的促生及其黑胫病的诱导抗性评价

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  • 国家科技期刊平台
  • NETL
  • NSTL
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为研究哈茨木霉菌(Trichoderma harzianum)对烟草促生作用及由烟草疫霉菌(Phytophthora nicotianae)引起的烟草黑胫病的诱导抗性分子机制，对5株木霉菌株进行筛选。结果表明，哈茨木霉ACCC 32808(TH32808)菌株对烟草疫霉菌的抑制性较强，抑制率为62。00%。进一步研究TH32808对不同烟草品种的促生作用及对烟草黑胫病的生物防治诱导抗性，结果表明，在盆栽条件下，TH32808对不同烟草品种的促生作用不同，其中红花大金元的植株鲜重显著增加8。30%，对黑胫病害的防治效果可达51。74%;红花大金元和K326的过氧化氢酶活性在第4天时较高，分别为对照的1。42和2。15倍，过氧化物酶活性在第2天时较高，分别为对照的2。49和2。29倍，超氧化物歧化酶活性在第4天较高，分别为对照的1。17和1。15倍。TH32808可诱导烟草对黑胫病的抗性，关键信号分子水杨酸、茉莉酸和乙烯代谢途径的关键基因NPR1、PDF1。2、EFE和NtSGT1均呈上调表达趋势。研究结果为烟草黑胫病的生物防治和诱导抗性研究提供了生防真菌资源和理论依据。
Evaluation of the Growth-promoting Effect of Trichoderma harzianum on Tobacco and Its Induced Resistance to Black Shank Disease
To investigate the growth-promoting effects of Trichoderma harzianum on tobacco and its induced resistance mechanisms against tobacco black shank disease caused by Phytophthora nicotianae,5 Trichoderma strains were screened.The results showed that the strain T.harzianum ACCC 32808(TH32808)was demonstrated the strongest inhibition(62.00%)among 5 strains of Trichoderma.The growth-promoting effects of TH32808 on different tobacco varieties and its induced resistance against tobacco black shank disease were assessed.The results showed that,under pot condition,TH32808 exhibited different ability on promoting the growth of different tobacco varieties.Under TH32808 treatment,the fresh weight of Honghuadajinyuan increased by 8.30%,the control efficacy against black shank disease in Honghuadajinyuan was 51.74%.In Honghuadajinyuan and K326,the catalase activity peaked on the 4th day,reaching 1.42 and 2.15 times that of CK,the peroxidase activity peaked on the 2nd day,being 2.49 and 2.29 times that of CK,the superoxide dismutase activity peaked on the 4th day,reaching 1.17 and 1.15 times that of CK,respectively.Moreover,TH32808 could induce resistance against black shank disease,key genes involved in the metabolic pathways of salicylic acid,jasmonic acid and ethylene,such as NPR1,PDF1.2,EFE and NtSGT1,all exhibited an upregulation trend.Above results provided fungal resources and theoretical basis for the biological control and induced resistance research against black shank disease in tobacco.

Trichoderma harzianumgrowth-promoting effectblack shank disease in tobaccoinduced resistancebiological control

常峻嘉、盖佳鑫、陶刚、莫转龙海

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贵州民族大学生态环境工程学院,贵阳 550025

中国农业科学院农业资源与农业区划研究所,北京 100081

哈茨木霉 促生作用 烟草黑胫病 诱导抗性 生物防治

贵州民族大学科研基金项目2023贵州民族大学大学生创新创业国家级训练计划项目国家自然科学基金项目

GZMUZK[2023]YB2220231067205131860520

2024

10.13304/j.nykjdb.2024.0225

中国农业科技导报
中国农村技术开发中心

中国农业科技导报

CSTPCD北大核心
影响因子：1.252
ISSN：1008-0864
年,卷(期)：2024.26(10)