Impact of Nitrogen Application Rate on Metabolism and Quality of Upper Leaves of Yunyan 121
To enhance the quality and usability of tobacco upper leaves,and screen suitable nitrogen application rate for Yunnan 121 in the Qujing tobacco-growing region,the field experiments were conducted with nitrogen application rates of 82.5,105.0 and 127.5 kg·hm-2.Agronomic traits were recorded under different treatments,the ultrastructure of mature upper tobacco leaves was examined,and the metabolomic was analyzed.After curing,the indicators such as external quality,chemical composition,sensory quality,etc.,were measured.The growth status and upper leaf quality of Yunnan 121 under different nitrogen application rates were comprehensively evaluated.The results showed that there was no significant difference on the growth of tobacco plants under 3 nitrogen application rate treatments,with the lowest comprehensive disease incidence observed in 105.0 kg·hm-2 nitrogen treatment.With increasing nitrogen application rates,the degradation of chloroplasts in the upper leaves of Yunnan 121 slowed down,and the volumes of starch granules and eosinophilic granules gradually increased,with the highest accumulation of eosinophilic granules of leaves in 105.0 kg·hm-2 nitrogen treatment.Nitrogen application levels influenced the metabolic patterns of upper leaves in Yunnan 121,primarily focusing on the pathways of oxalic acid and phenylpropane metabolism.Under the 105.0 kg·hm-2 nitrogen treatment,the post-curing appearance quality,chemical composition,sensory quality,and economic traits of upper leaves in Yunnan 121 were superior to the other 2 nitrogen application levels.Consequently,it should be considered that pure nitrogen of 105.0 kg·hm-2 could effectively improve the quality and usability of upper leaves in Yunnan 121 in the Qujing tobacco-growing region.
Yunyan 121upper leavesnitrogen application ratequality of roasted tobaccometabolomicsultrastructuralagronomic traits
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