Application of UV-visible Spectral Library in Recessive Screening of Pesticide Fungicides
To establish the ultraviolet-visible(UV-VIS)spectral library to quickly and accurately retrieve the hidden components added in the pesticide fungicide,with acetonitrile and water as the mobile phase,the UV-VIS spectrograms of 98 conventional bactericide standards were separated and extracted by gradient elution using a high-performance liquid chromatograph,and the UV-VIS spectrograms of the standard substances were used to establish the UV-VIS spectrogram library.The target compound was matched according to the spectral similarity and the retention time of the standard sample,so as to retrieve the hidden components added in the pesticide preparation.2 bactericides were used to verify the accuracy of the spectral library retrieval,and the results showed that the accuracy of the spectral library retrieval reached 100%.This method had the advantages of high accuracy,easy operation and low cost.
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