紫外-可见光谱库在农药杀菌剂隐性排查中的应用
Application of UV-visible Spectral Library in Recessive Screening of Pesticide Fungicides
陈颖 1刘小锋 1陈一萍 1廖丽萍 1傅洪涛1
作者信息
- 1. 湖南化工研究院有限公司,国家农药创制工程技术研究中心,长沙 410014;湖南化肥农药质量监督检验授权站,长沙 410014
- 折叠
摘要
为建立紫外-可见光谱库以期能够快速准确的检索农药杀菌剂中添加的隐性成分,通过高效液相色谱仪,以乙腈和水做为流动相,采用梯度洗脱分离及提取98种常规杀菌剂标准物质的紫外-可见光谱图,以标准物质的紫外-可见光谱图来建立紫外-可见光谱库.根据光谱的相似度和标样的保留时间来匹配目标化合物,从而检索出农药制剂中添加的隐性成分.通过2个杀菌剂来验证谱库检索的准确性,结果显示,谱库检索的正确性达100%.该方法具有检测目标化合物准确率高、操作简单、易学、成本较低等优点.
Abstract
To establish the ultraviolet-visible(UV-VIS)spectral library to quickly and accurately retrieve the hidden components added in the pesticide fungicide,with acetonitrile and water as the mobile phase,the UV-VIS spectrograms of 98 conventional bactericide standards were separated and extracted by gradient elution using a high-performance liquid chromatograph,and the UV-VIS spectrograms of the standard substances were used to establish the UV-VIS spectrogram library.The target compound was matched according to the spectral similarity and the retention time of the standard sample,so as to retrieve the hidden components added in the pesticide preparation.2 bactericides were used to verify the accuracy of the spectral library retrieval,and the results showed that the accuracy of the spectral library retrieval reached 100%.This method had the advantages of high accuracy,easy operation and low cost.
关键词
高效液相色谱仪/杀菌剂/紫外-可见光谱库/农药Key words
high-performance liquid chromatography/bactericide/ultraviolet-visible spectrum library/pesticide引用本文复制引用
出版年
2024
国家科技期刊平台
NSTL
万方数据