Relationship Between DHN Melanin and Formation of Appressorium Turgor Pressure of Setosphaeria turcica
[Objective] The objective of this study is to discuss the effect of intracellular DHN melanin ofSetosphaeria turcica on the appressorium turgor pressure generated and elucidate the invasive mechanism of S. turcica. [Method] In this research, the best conditions for appressorium formation of S. turcica were determined by inducing appressorium production. The appressorium cell wall pore sizes and turgor pressures of wild-type isolate 01-23 and melanin-deficient strains ΔSt3hnr of S. turcica were measured by a solute exclusion technique and incipient-cytorrhysis technique. [Result] The appressorium pore size of wild-type isolate 01-23 was 2.1-2.7 nm and it had a 5.4 MPa turgor pressure, while it was shown a range of 2.7-3.3 nm in melanin-deficient strainsΔSt3hnr and a lower pressure about 4.1 MPa. The results indicated that appressorium without melanin could not form a high turgor pressure resulting in losing the ability for penetrating. [ Conclusion] It is suggested that the melanin layer is significant in accumulation of excess turgor pressure and the turgor pressure of S. turcica play a major role in substance penetration.
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万方数据
维普
