Antimicrobial Action of an Endophytic Fungus from Sophora flavescens and Structure Identification of Its Active Constituents
[Objective] The objective of this study is to isolate the endophytic fungi from Sophora flavescens and define its antimicrobial action. Also the identification of the category and structure of the active substance fermented from Sophora flavescens was conducted. [Method] Tissue isolation and selective culture medium method was used to isolate the endophytic fungi. Plate method was applied to measure the antimicrobial characters. Eight kinds of solvent system was used to define its polar. Column chromatography and freeze drying, TLC biological autoradiography and HPLC were employed to isolate and purify, trace, and measure the purity, respectively. The structure was analyzed using 1H-NMR, 13C-NMR and LC-MS. [Result] An endophytic fungus strain was isolated from Sophora flavescens seed, numbered BS001. It inhibited the growth of Phytophthora capsici, Colletotrichum coccodes, Sclerotinia sclerotiorum, and Alternaria mali. The inhibition to Colletotrichum coccodes was the biggest with the antibacterial diameter of 2.734 cm. After isolating and purifying the fermentation liquor of BS001, the structure of bacteriostatic active composition was 6,7-(2'E)dibutenyl-5,8-dihydroxy-(Z)-cyclooct-2-ene-l,4-dione, which identified by spectroscopy. [Conclusion] An endophytic fungus, BS001, which has inhibition to some pathogens, was got from Sophora flavescens. Also a new antimicrobial substance 6,7-(2'E)dibutenyl-5,8-dihydroxy-(Z)-cyclooct-2-ene-l,4-dione was extracted from fermented broth of BS001. This antmicrobial substance can be used for development of biopharmaceuticals and biopesticides.
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