Isolation of Dominant Actinomycetes from Cherry Orchard Soil and Their Functions in Bio-Control and Plant Promotion
[Objective] The dominant actinomycetes in cherry orchard soil were isolated and collected at different growth phases to study their application potential in biocontrol and plant growth promotion.[Method] All isolates were assayed for antagonistic activity,fermentation activity in vitro experiments to analyze their antagonistic and growth promoting function.Strain F4-5 with good microorganism inhibiting effect and plant growth promoting functions was explored through 16S rDNA sequence analysis to ascertain its taxonomic status.[Result] There were 32 dominant actinomycetes were isolated from Cerasus avium (L.)Moench / C.sachalinensis (L.) Kom.Rhizosphere soil at different growth stages.The number of soil actinomycetes in August was the highest compared with that in May and November.The isolates from different growth stages also varied in their microorganism inhibiting and plant growth promoting capabilities.In general,isolates from August 2010 expressed obvious effect on antagonistic pathogen and plant growth promotion.Among all the 32 isolates,strain F4-5 showed a significant inhibitive effect on 4 tested bacteria including Staphylococcus aureus,Escherichia coli,Bacillus subtilis and B.cereus,as well as 3 tested fungi including Trichoderma viride,Rhizopus stolo and Aspergillus niger,exhibiting a broad-spectrum antibiosis.In the experiment of seeding growth of Malus baccata (L.) Brokh.,strain F4-5 also displayed a positive effect on seed germination and root growth,increased by 201.1％ and 43.5％,respectively,compared with the control,indicating both antibiosis and plant promoting effects.In addition,strain F4-5 was identified as Streptomyces flavogriseum,according to the morphological characteristics,cultural characteristics and 16S rDNA sequence analysis.[Conclusion] The actinomycete number,distribution,antibiotic and seed germination promotion in Cerasus avium (L.) Moench / C sachalinensis (L.) Kom.Rhizosphere soil varied greatly at different stages.The proper strain F4-5 (S.flavogriseum) screened from 32 actinomycete strains showed a good probiotic role and seed germination promotion.
国家科技期刊平台
NSTL
万方数据
维普
