Expression and Genotype Analysis of VvmybA1 in Chinese Wild Grapes and Interspecific Hybrids
[Objective] The aim of this study is to detect the genotypes of Chinese wild grapes and the hybrids of V.amurensis and V.vinifera and compare the VvmybA1 gene sequences in different wild grapes,reveal the molecular mechanism of no anthocyanin collected in white-fruited Xiongyuebaiputao by analysising the expression and genotype of VvmybA 1 in the interspecific hybrids (V.amurensis × V.vinifera).[Method] The specific primers were designed to detect the genotype of different wild grapes by the means of molecular cloning and absolute quantitative real-time PCR.Using Real-time PCR methods,analysis the expression of VvmybA1 and UFGTin the the hybrids of V.amurensis and V.vinifera during veraison.[Result] ①There was no VvmybAla allele detected in Chinese wild grapes.②Comparing sequences of VvmybA1 gene in the different species of Chinese wild grapes with thoses of V.vinifera,extensive base deletion,insertion and substitution existt in promotor region,intron region and the third coding exon,and exhibits rich genetic diversity between species of Chinese wild grapes.Furthermore,there are se.veral unique bases or mutations in the VvmybA1 gene of wild grapes which could be selected to be the molecule marker of distinguishingdifferent vitis species and cultivars.③VvmybAla allele has been discovered in Suxiaohong and the hybrids of V.amurensis × V.vinifera,including Gongniang 1,Beihong and Xiongyuebaiputao.In addition,Xiongyuebaiputao is the homozygous genotype of VvmybAla.④ RT-PCR analysis showed high expression of VvmybA1 and UFGT in Gongniang 1 during veraison,but theydid not express in Xiongyuebaiputao during the entire mature period.[Conclusion] The result proved that VvmybA la allele didn't exist in Chinese wild grapes but existed in V.vinifera and its interspecific hybrids uniquely.High consanguinity was speculated between Suxiaohong and V.vinifera.Different VvmybA1 gene sequences of species in Vitis were obtained and compared with each other.The reason why Xiongyuebaiputao has no anthocyanin collected is that the homozygous genotype of VvmybAla lead to the no expression of VvmybAl.
Chinese wild grapesVvmybA1anthocyaninsgenotypingskin color
国家科技期刊平台
NSTL
万方数据
维普
