[Background]As one of the most important periods in plant growth and development,the flowering period directly affects fruit ripening and seed development.circRNAs are a class of covalent closed-loop RNA molecules that are ubiquitous in eukaryotic cells and play an important role in the regulation of tomato development and stress response.However,the current circRNA studies on tomato mainly focus on fruit and leaves,and there is a lack of systematic studies on tomato circRNA at flowering stage.[Objective]Identification and analysis of circRNs in flowering tomato could be of great significance for the functional study of miRNA and circRNA in tomato,and also layed a foundation for the study of tomato growth,development and stress response mechanism.[Method]circRNA sequencing was performed on 3 tissue samples of flowers,roots and leaves of flowering tomato plants,with 3 replicates for each sample.circRNAs were identified and their basic characteristics were analyzed.The cycle-forming ability of tissue-specific circRNAs was screened,and the host genes of identified circRNAs were analyzed by GO analysis and KEGG analysis.The mode and site of action of circRNAs were predicted and analyzed by bioinformatics methods to construct a potential circRNA-miRNA-mRNA interaction regulatory network in response to tomato growth and development.[Result]A total of 532 circRNAs were obtained by high-throughput sequencing,83%of which were exon types.The distribution of circRNA in each chromosome of flowering tomato was uneven,among which chromosome 1 produced the most circRNAs and chromosome 5 produced the least.circRNAs differentially expressed in flower,leaf and root tissues of flowering tomato showed that 79 circRNAs were differentially expressed in flower and leaf,133 circRNAs were differentially expressed in flower and root,and 132 circRNAs were differentially expressed in leaf and root tissues.Among them,14 circRNAs were differentially expressed in flower,leaf and root tissues.The cyclization ability of 8 circRNAs randomly selected from 14 differentially expressed circRNAs was tested,and the results showed that all 8 circRNAs had cyclization ability.GO analysis and KEGG analysis showed that circRNAs in flowering tomato were mainly related to the binding of nucleic acids,proteins and other small molecules,as well as the synthesis and metabolism of various biological macromolecules.Finally,the tomato circRNA-miRNA-mRNA interaction regulatory network composed of 14 circRNAs,10 miRNAs and 136 mRNAs was constructed.[Conclusion]A total of 342 tissue-specific circRNAs were identified,among which 14 were significantly expressed,and 8 were successfully identified.A circRNA-miRNA-mRNA interaction regulatory network was constructed for tomato at flowering stage.This study laid a foundation for the subsequent research of circRNA in flowering period.
国家科技期刊平台
NSTL
万方数据
维普
