Research Advances of Map-Based Cloning Genes in Cotton
Map-based cloning is a classical and effective method to identify candidate genes for specific phenotypic variants.Map-based cloning of functional genes plays important roles in the innovative utilization of germplasm resources,molecular design breeding and improving breeding efficiency.In recent years,the whole-genome sequencing of Gossypium raimondii,Gossypium arboreum,Gossypium hirsutum,and Gossypium barbadense has been completed and improved.Map-based cloning has entered into a crucial period.In 2016,the dominant glandless gene Gl2e(GoPGF)was the first map-based cloning gene in cotton.So far,20 qualitative traits genes and 5 quantitative traits genes have been identified by map-based cloning technology.In this paper,research progress was systematically reviewed in fiber,gland,nectary,leaf type,plant architecture,plant color,and fertility in terms of gene symbols,names,chromosomal positioning,and candidate genes.Moreover,map-based cloning strategies were systematically reviewed in mapping populations and bulked segregate analysis-sequencing(BSA-seq).With the reduction of sequencing cost and utilization of BSA-seq,it is believed that more and more genes will be cloned by map-based cloning technology.In addition,transformation and genome editing have been successfully used to evaluate the function of the candidate gene in the target interval.It is believed that map-based cloning could provide a theoretical basis and genetic resources for molecular design breeding in cotton.
国家科技期刊平台
NSTL
万方数据
维普
