Changes of Endogenous Hormones and Polyamines During Ovule Development of Stenospermocarpic Seedless Grape
[Objective]By exploring the effects of endogenous hormones and polyamines on embryo development during the ovule development of seedless grape,this study provided a theoretical basis for promoting embryo development by spraying exogenous hormones before anthesis and adding exogenous hormones into the medium in vitro culture of ovule.[Method]In this study,the European grape(Vitis vinifera L.)variety Jingxiu and its F1 generation stenospermocarpic seedless variety Qinxiu were used as test materials.The content of endogenous hormones and polyamines in ovule of fruit at different developmental stages were compared by high performance liquid chromatography mass spectrometry(HPLCMS)and ultra performance liquid chromatography(UPLC).[Result]The content of IAA,ZT,ZR,and iPAS reached their highest values at 39 DAF(days after flowering)for Jingxiu and 42 DAF for Qinxiu,and the highest values of the former were higher than those of the latter(more than 1.5 times).For the content of JA and SA,Jingxiu started to rise sharply at 36 DAF(2 000 ng g-1 and 6 500 ng g-1,respectively),and reached the highest values at 39 DAF(6 500 ng·g-1 and 10 000 ng·g-1,respectively),and then,which were declining and remaining at a high level at 42-45 DAF,while Qinxiu was declining and remaining at a low level from 36 DAF(3 500 ng·g-1 and 3 000 ng·g-1,respectively).The ACC content of Jingxiu was almost 0 ng·g-1 at 39 DAF,then increased rapidly and reached its highest value(1 200 ng·g-1)at 45 DAF,while Qinxiu reached its highest value(900 ng·g-1)at 39 DAF,then declined to 0 ng·g-1 at 42 DAF,and remained at a very low level.The ABA content of Jingxiu was almost 0 ng·g-1 from 36 to 45 DAF,while that of Qinxiu rose sharply from 0 ng·g-1 at 39 DAF to the highest value(900 ng·g-1)at 42 DAF.The trends of Put(putrescine),Spm(spermine),and Spd(spermidine)were similar for the two varieties,but Jingxiu was consistently higher than Qinxiu.The highest values of Put and Spd were 42 DAF for Jingxiu and 39 DAF for Qinxiu,except for the highest values of Spm,which were all found at 42 DAF.[Conclusion]The lower content of growth promoting substances(IAA,CTK,GA3,ACC,JA,SA,Put,Spd,and Spm)and the higher content of growth inhibiting substances(ABA),and lower ratios of(IAA+GA3)/ABA,(IAA+ZT+GA3)/ABA,Spm/PAs,(Spd+Spm)/PAs,and(Spd+Spm)/Put in the ovules of the seedless variety Qinxiu compared with the seeded variety Jingxiu might be one of the main reasons for seedless grape embryo abortion.Therefore,in the process of seedless grape embryo rescue,the embryo abortion could be inhibited by spraying before flowering or adding to media a certain concentration of growth promoting substances.
国家科技期刊平台
NSTL
万方数据
维普
