[Objective]To clarify the requirements of oilseed rape cultivars characterized by high-oil content for nitrogen(N),phosphorus(P),and potassium(K)nutrients,the dynamic nutrient accumulation rates of cultivars with different oil content were compared,so as to provide a theoretical basis on precise nutrient management for realizing high oil yield potential.[Method]The field and pot experiments were carried out during 2019-2021 oilseed rape growth seasons to monitor the dynamic changes in biomass,NPK content and accumulation rates of oilseed rapes cultivars with high oil content(Zhongyouza 19,Z19)and conventional oil content control cultivars(Huayouza 12,H12,and Huayouza 62,H62).And then the differences in nutrient utilization efficiency were analyzed.[Result]The results of two-year experiments showed that the seed yield of Z19 was obviously lower than that of H62 by 8.5%-20.4%,but there was little difference in seed yield between Z19 and H12.However,the oil content of Z19 was significantly higher than those of H12 and H62(10.1%-26.7%).By monitoring the dynamic biomass and nutrient content in the field and pot traits during the 2020/2021 growth seasons,it was claimed that the biomass of Z19 was lower than that of H62,which was higher than that of H12 at podding and mature stage with different degrees,while there was minor difference in biomass between Z19 and H12 before podding stage.The N content of Z19 was significantly lower than H12 and H62 during growth in pot experiment and after flowering stage in field experiment.And K content of Z19 was higher than H12 and H62 during growth.However,there was no consistent changes in P content between field and pot experiments among three cultivars.Consequently,the N accumulation rates of Z19 were lower than that of H62 by 19.2%-29.0%and lower than that of H12 by 9.8%-13.1%in pot experiment.The K accumulation rate of Z19 was higher than H12 by 7.4%-39.2%except for the flowering stage.Moreover,the P accumulation rate of Z19 was between those of H62 and H12,which was remarkably higher than that of H12 in the field and significantly lower than that of H62 in the pot.The N utilization efficiency and oil production efficiency of Z19 were significantly higher than those of H12 and H62.Moreover,the K utilization efficiency of Z19 was lower than that of H12 and H62,but the K oil production efficiency of Z19 was close to that of H12 and H62.There was minor difference in P utilization efficiency among 3 cultivars,but the P oil production efficiency of Z19 was significantly higher than that of H12.15N labeling indicated that there was no obviously difference in N fertilizer use efficiency among three cultivars until flowering stage,after which N fertilizer use efficiency of Z19 was significantly lower than that of H12 and H62.[Conclusion]In summary,high-oil content cultivar,Z19,had a higher demand for K and a lower demand for N with a higher N utilization efficiency.In agricultural production,K fertilizer could be supplemented for high-oil content cultivar,and less N fertilizer could be applied in comparison to the conventional oilseed rape cultivars.