首页|冠层活性相关性状全基因组关联分析及其对产量性状遗传效应的解析

冠层活性相关性状全基因组关联分析及其对产量性状遗传效应的解析

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[目的]冠层活性是反映作物生长发育的重要指标,发掘小麦冠层活性相关基因并分析其与产量性状的关系,为解析产量遗传结构和辅助育种提供理论支撑.[方法]以 166 份国内外小麦品种为材料,将其种植于河南安阳和安徽濉溪,结合已构建的包含326 570个来源于小麦90K和660K SNP芯片标记的高密度整合物理图谱,对苗期和花后10 d归一化植被指数(NDVI-S和NDVI-10)及花后 10 d旗叶叶绿素含量(Chl-10)进行关联分析,并与前期利用相同材料得到的产量相关性状结果进行比较.[结果]NDVI-S、NDVI-10和Chl-10在基因型、环境及基因型×环境互作间变异方差均达极显著(P＜0.01)差异,广义遗传力(h2 b)分别为 0.81、0.81 和 0.91.分别检测到 13、12 和 15 个与NDVI-S、NDVI-10 和Chl-10 显著相关的位点,其中,有12、11和12 个为新位点,5个位点与2个以上性状有关.166份小麦品种含有NDVI-S、NDVI-10和Chl-10优异等位基因数变幅分别为4-11、3-11和4-12,且随着优异等位基因数目增加其对应表型值呈递增趋势.NDVI-S与千粒重、粒长和粒宽显著正相关(P＜0.01);Chl-10 与产量和旗叶宽显著正相关(P＜0.01),与单位面积穗数、株高和穗下节长显著负相关(P＜0.01).检测到7个同时与产量和冠层活性相关的多效性位点.[结论]NDVI-S可用于品种产量性状的选择,检测到的稳定位点和多效性位点可在开发标记后用于育种材料的检测.
Genome-Wide Association Studies for Canopy Activity Related Traits and Its Genetic Effects on Yield-Related Traits
[Objective]Canopy activity is an important indicator of wheat growth and development.Identification the loci for canopy activity related traits and their relationships with grain yield(GY)related traits can provide theoretical support for the dissection of genetic structure of yield trait and assisted wheat breeding.[Method]A total of 166 wheat varieties originating from both domestic and international sources were planted in Anyang of Henan province and Suixi of Anhui province in cropping seasons.With the integrated physical map containing 326 570 SNP markers from the wheat 90K and 660K chips,genome-wide association studies for normalized difference vegetation index at seedling stage(NDVI-S)and 10 days after flowering(NDVI-10),and chlorophyll content in flag leaf at 10 days after flowering(Chl-10)were carried out.The results were compared with the previous study for GY related traits using the same material.[Result]Analysis of variance(ANOVA)showed highly significant effects(P＜0.01)of genotypes,environments and genotype×environment interactions on NDVI-S,NDVI-10 and Chl-10,with broad-sense heritabilities(h2 b)of 0.81,0.81 and 0.91,respectively.Thirteen,12 and 15 loci were detected to be significantly correlated with NDVI-S,NDVI-10 and Chl-10,respectively,among which 12,11 and 12 were new,and five loci were associated with two or more traits.The number of favorable alleles for NDVI-S,NDVI-10 and Chl-10 ranged from 4 to 11,3 to 11 and 4 to 12,respectively,in the 166 wheat varieties,and the phenotypic values increased with the accumulation of favorable alleles.NDVI-S showed significant(P＜0.01)and positive correlations with thousand-kernel weight,kernel length and kernel width.Chl-10 was significant positively correlated with GY and flag leaf width(P＜0.01),whereas significant negatively correlated with spike number per unit area,plant height and uppermost internode length(P＜0.01).Seven pleiotropic loci were detected co-related with both GY and canopy activity related traits.[Conclusion]NDVI-S can be directly used for selection of yield traits.The stable and pleiotropic loci detected in this study can be used for marker-assisted selection.

Triticum aestivumcanopy activitygrain yieldnormalized difference vegetation indexchlorophyll contentgenome-wide association studies

李法计、程敦公、余晓丛、闻伟锷、刘金栋、翟胜男、刘爱峰、郭军、曹新有、刘成、宋健民、刘建军、李豪圣

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山东省农业科学院作物研究所/小麦玉米国家工程研究中心/农业农村部黄淮北部小麦生物学与遗传育种重点实验室/山东省小麦技术创新中心/济南市小麦遗传改良重点实验室,济南 250100

菏泽学院农业与生物工程学院(牡丹学院),山东菏泽 274015

遵义医科大学细胞生物学教研室,贵州遵义 563099

中国农业科学院作物科学研究所,北京 100081

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小麦 冠层活性 产量 归一化植被指数 叶绿素含量 关联分析

山东省重点研发计划(重大科技创新工程)泰山产业领军人才工程国家现代农业产业技术体系2023年山东省农业科学院科技创新工程济南市"新高校20 条"资助项目

2021LZGC009LJNY202006CARS-03-6CXGC2023A01202228067

2024

10.3864/j.issn.0578-1752.2024.04.001

中国农业科学
中国农业科学院

中国农业科学

CSTPCD北大核心
影响因子：1.899
ISSN：0578-1752
年,卷(期)：2024.57(4)
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