[Objective]Canopy activity is an important indicator of wheat growth and development.Identification the loci for canopy activity related traits and their relationships with grain yield(GY)related traits can provide theoretical support for the dissection of genetic structure of yield trait and assisted wheat breeding.[Method]A total of 166 wheat varieties originating from both domestic and international sources were planted in Anyang of Henan province and Suixi of Anhui province in cropping seasons.With the integrated physical map containing 326 570 SNP markers from the wheat 90K and 660K chips,genome-wide association studies for normalized difference vegetation index at seedling stage(NDVI-S)and 10 days after flowering(NDVI-10),and chlorophyll content in flag leaf at 10 days after flowering(Chl-10)were carried out.The results were compared with the previous study for GY related traits using the same material.[Result]Analysis of variance(ANOVA)showed highly significant effects(P＜0.01)of genotypes,environments and genotype×environment interactions on NDVI-S,NDVI-10 and Chl-10,with broad-sense heritabilities(h2 b)of 0.81,0.81 and 0.91,respectively.Thirteen,12 and 15 loci were detected to be significantly correlated with NDVI-S,NDVI-10 and Chl-10,respectively,among which 12,11 and 12 were new,and five loci were associated with two or more traits.The number of favorable alleles for NDVI-S,NDVI-10 and Chl-10 ranged from 4 to 11,3 to 11 and 4 to 12,respectively,in the 166 wheat varieties,and the phenotypic values increased with the accumulation of favorable alleles.NDVI-S showed significant(P＜0.01)and positive correlations with thousand-kernel weight,kernel length and kernel width.Chl-10 was significant positively correlated with GY and flag leaf width(P＜0.01),whereas significant negatively correlated with spike number per unit area,plant height and uppermost internode length(P＜0.01).Seven pleiotropic loci were detected co-related with both GY and canopy activity related traits.[Conclusion]NDVI-S can be directly used for selection of yield traits.The stable and pleiotropic loci detected in this study can be used for marker-assisted selection.
关键词
小麦/冠层活性/产量/归一化植被指数/叶绿素含量/关联分析
Key words
Triticum aestivum/canopy activity/grain yield/normalized difference vegetation index/chlorophyll content/genome-wide association studies
维普
万方数据