Genetic Analysis of Leaf Wrinkling Traits in Non-Heading Chinese Cabbage
[Background]Non-heading Chinese cabbage(Brassica campestris L.ssp.chinensis(L.)Makino),a member of the Cruciferae family,is a leafy vegetable widely cultivated in the southern regions of China.Among leaf traits,the leaf wrinkling is a significant agronomic characteristic of non-heading Chinese cabbage.This trait not only determines the visual marketability of non-heading Chinese cabbage,but also significantly affects its yield,nutritional quality,stress resistance,and photosynthetic activity of the leaves.However,there is currently no clear understanding of the genetic and formation mechanisms of this trait,which limits efficient genetic improvement of this trait.[Objective]The objective of this study was to quantitatively describe the leaf wrinkling trait of non-heading Chinese cabbage,to explore its genetic and formation mechanisms,and to enhance the efficient selection and regulation of this trait,so as to consequently guide breeding efforts for non-heading Chinese cabbage.[Method]Six distinct F2 populations were constructed using Tacai(Brassica campestris ssp.chinensis var.rosularis Tsen et Lee),which with conspicuous leaf wrinkling in non-heading Chinese cabbage as one of the parents.The leaf wrinkling trait of F2 plants was quantified during the rosette stage using the indicator"number of bulges in the maximum leaf".A genetic segregation analysis method for quantitative traits,incorporating both major gene and polygenic inheritance,was employed to investigate the genetic principles governing this trait.[Result]Significant variation was found among individual plants in the F2 population regarding leaf wrinkling traits,manifesting as continuous changes.The distribution curve demonstrated a normal or skewed normal distribution,suggesting that leaf wrinkling in non-heading Chinese cabbage was a quantitative trait influenced by multiple genes.Genetic analysis of these traits was then conducted utilizing a major gene+polygene genetic model calculation method.The result showed that the optimal genetic model for leaf wrinkling in non-heading Chinese cabbage was the 2MG-ADI gene model,with a major gene heritability of 99.08%.The first major gene's dominant effected mitigates wrinkling,while the second major gene's dominant effect intensified it.Both major genes exhibited additive effects,deepening wrinkling.Additive×additive and additive×dominant interaction effects reduced wrinkling,whereas dominant×additive and dominant×dominant interaction effects enhanced the traits.[Conclusion]The leaf wrinkling trait in non-heading Chinese cabbage was a quantitative characteristic governed by two pairs of major genes,with these major genes predominantly influencing its regulation.
non-heading Chinese cabbageleaf wrinkling traitquantitative traitTacaimain gene plus polygene genetic analysis
国家科技期刊平台
NSTL
万方数据
