Effect of Rice Varieties on the Preference of Nilaparvata lugens to Rice Plants Infested by Chilo suppressalis
[Objective]It has been reported that Nilaparvata lugens(brown planthopper,BPH)exhibits a preference of endangering rice plants infested by Chilo suppressalis(striped stem borer,SSB).However,it remains unclear whether this phenomenon is influenced by the developmental stage of N.lugens and the rice variety.This study aims to investigate the effects of rice varieties and developmental stages of N.lugens on the preference for C.suppressalis-infested rice plants,as well as how volatiles mediate this behavior,so as to provide a scientific basis for formulating green pest control strategies.[Method]Through indoor choice experiments,the attraction of different developmental stages of N.lugens(including 3rd-4th instar nymphs,newly emerged females,gravid females)to nine rice varieties,including Zhongzheyou 8,both damaged and undamaged by C.suppressalis,was observed.Based on these observations,representative varieties showing significant differences in attraction were selected for further analysis.RT-qPCR was used to detect changes in the expression of volatile synthesis-related genes in three rice varieties(Zhongzheyou 8,9311,and Minghui 63)following pest infested.[Result]Among the nine rice varieties,different developmental stages of N.lugens consistently showed a preference for rice plants infested by C.suppressalis,although this preference varied among rice varieties.For instance,newly emerged female N.lugens exhibited a significantly higher preference for Minghui 63 compared to Yongyou 538.Gene expression analysis revealed a significant increase in the expression levels of volatile synthesis-related genes OsCAS,OsLIS,OsHPL3,and OsRCI-1 in response to C.suppressalis infestation.However,when different developmental stages of N.lugens were co-infested with C.suppressalis,the expression levels of these genes could either increase or decrease compared to plants infested only by C.suppressalis,with specific effects depending on the developmental stage of N.lugens.[Conclusion]The preference of N.lugens for rice plants infested by C.suppressalis was commonly observed across the nine tested rice varieties.However,significant differences in the degree of preference were noted among different developmental stages of N.lugens across these varieties.Notably,variations in preference among developmental stages of N.lugens were primarily observed in the selection of rice varieties Yongyou 538,Zhongzheyou 8,and Minghui 63 by newly emerged female.Infestation only by C.suppressalis or in conjunction with N.lugens resulted in increased expression levels of the genes OsCAS,OsLIS,OsHPL3,and OsRCI-1,indicating that pest infestation in rice plants can regulate the synthesis of rice volatiles,thereby influencing the host selection behavior of N.lugens.
万方数据
维普
