Preliminary verification of animal model of benign esophageal stricture caused by gastroesophageal reflux in rats
Objective To develop an experimental model of gastroesophageal reflux-induced esophageal stricture in rats and explore the mechanism of esophageal stricture.Methods A total of 30 male Sprague-Dawley(SD)rats by random number table method were randomly divided into three groups as follows:an operation+acid perfusion group,first the models of lower esophageal sphincter relaxation and hiatal hernia were made,and then the rats'esophagus were perfused with hydrochloric acid-pepsin;acid perfusion group,the rats'esophagus were directly perfused with hydrochloric acid-pepsin;and control group,rats'esophagus were perfused with normal saline.After 4 weeks of continuous perfusion,the esophageal mucosal injury of SD rats in each group were observed,and the concentrations of inflammatory cytokines[tumor necrosis factor-α(TNF-α),interleukin(IL)-1 β and IL-18]in esophageal tissues were detected by enzyme-linked immunosorbent assay.Results In the operation+acid perfusion group,esophageal stricture was formed in 2 SD rats,but no esophageal stenosis was found in the acid perfusion group and the control group.The body weight of rats in the operation+acid perfusion group and the acid perfusion group were lower than that in the control group(P＜0.05).The esophageal mucosal injury scores of rats in the operation+acid perfusion group and the acid perfusion group were higher than that in the control group(P＜0.001),and the operation+acid perfusion group was higher than that in the acid perfusion group(P=0.014).The concentrations of TNF-α,IL-1β and IL-18 in esophageal tissues were higher in the operation+acid perfusion group and the acid perfusion group than that in the control group(P＜0.001),and the operation+acid perfusion group was higher than that in the acid perfusion group(P＜0.001).Conclusions The anti-reflux barrier is an important part of preventing gastroesophageal reflux disease.The destruction of anti-reflux barrier,hydrochloric acid-pepsin perfusion and inflammatory cytokines jointly induced esophageal inflammation and injury,and even caused esophageal stricture.
NETL
NSTL
万方数据
